TY  - JOUR
ID  - discovery176618
UR  - http://dx.doi.org/10.1371/journal.pgen.0030020
N2  - Imprinted genes undergo epigenetic modifications during gametogenesis, which lead to transcriptional silencing of either the maternally or the paternally derived allele in the subsequent generation. Previous work has suggested an association between imprinting and the products of retrotransposition, but the nature of this link is not well defined. In the mouse, three imprinted genes have been described that originated by retrotransposition and overlap CpG islands which undergo methylation during oogenesis. Nap1l5, U2af1-rs1, and Inpp5f_v2 are likely to encode proteins and share two additional genetic properties: they are located within introns of host transcripts and are derived from parental genes on the X chromosome. Using these sequence features alone, we identified Mcts2, a novel candidate imprinted retrogene on mouse Chromosome 2. Mcts2 has been validated as imprinted by demonstrating that it is paternally expressed and undergoes promoter methylation during oogenesis. The orthologous human retrogenes NAP1L5, INPP5F_V2, and MCTS2 are also shown to be paternally expressed, thus delineating novel imprinted loci on human Chromosomes 4, 10, and 20. The striking correlation between imprinting and X chromosome provenance suggests that retrotransposed elements with homology to the X chromosome can be selectively targeted for methylation during mammalian oogenesis
IS  - 2
AV  - public
TI  - A screen for retrotransposed imprinted genes reveals an association between X chromosome homology and maternal germ-line methylation
SN  - 1553-7390
Y1  - 2007/02/09/
KW  - 10
KW  -  2
KW  -  A
KW  -  AND
KW  -  Animals
KW  -  ARTICLE
KW  -  Aspartic Endopeptidases
KW  -  Base Sequence
KW  -  CpG Islands
KW  -  DNA Methylation
KW  -  Exons
KW  -  Female
KW  -  FOR
KW  -  genetics
KW  -  Genomic Imprinting
KW  -  Human
KW  -  Humans
KW  -  Introns
KW  -  JOURNAL
KW  -  London
KW  -  metabolism
KW  -  Methylation
KW  -  Mice
KW  -  Molecular Sequence Data
KW  -  Nerve Tissue Proteins
KW  -  OF
KW  -  Oncogene Proteins
KW  -  Ovum
KW  -  Phosphoric Monoester Hydrolases
KW  -  Phylogeny
KW  -  Proteins
KW  -  Retroelements
KW  -  Sequence Analysis
KW  -  DNA
KW  -  Sequence Homology
KW  -  Nucleic Acid
KW  -  THE
KW  -  United Kingdom
KW  -  X Chromosome
JF  - PLoS Genetics
A1  - Wood, AJ
A1  - Roberts, RG
A1  - Monk, D
A1  - Moore, GE
A1  - Schulz, R
A1  - Oakey, RJ
N1  - © 2007 Wood et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This work was supported by the Wellcome Trust and the Biotechnology and Biological Sciences Research Council (RJO), the Generation Trust (AW), and the European Molecular Biology Organization (RS).
VL  - 3
ER  -