TY  - JOUR
TI  - VEGF guides angiogenic sprouting utilizing endothelial tip cell filopodia
EP  -  1177
AV  - public
Y1  - 2003/06/23/
ID  - discovery16410
N2  - Vascular endothelial growth factor (VEGF-A) is a major regulator of blood vessel formation and function. it controls several processes in endothelial cells, such as proliferation, survival, and migration, but it is not known how these are coordinately regulated to result in more complex morphogenetic events, such as tubular sprouting, fusion, and network formation. We show here that VEGF-A controls angiogenic sprouting in the early postnatal retina by guiding filopodial extension from specialized endothelial cells situated at the tips of the vascular sprouts. The tip cells respond to VEGF-A only by guided migration; the proliferative response to VEGF-A occurs in the sprout stalks. These two cellular responses are both mediated by agonistic activity of VEGF-A on VEGF receptor 2. Whereas tip cell migration depends on a gradient of VEGF-A, proliferation is regulated by its concentration. Thus, vessel patterning during retinal angiogenesis depends on the balance between two different qualities of the extracellular VEGF-A distribution, which regulate distinct cellular responses in defined populations of endothelial cells.
PB  - ROCKEFELLER UNIV PRESS
KW  - VEGF
KW  -  endothelial cell
KW  -  filopodia
KW  -  astrocyte
KW  -  migration
KW  -  proliferation
KW  -  VASCULAR-PERMEABILITY FACTOR
KW  -  GROWTH-FACTOR
KW  -  RETINAL VASCULATURE
KW  -  BRANCHING MORPHOGENESIS
KW  -  CEREBRAL-CORTEX
KW  -  BLOOD-VESSELS
KW  -  TUMOR-CELLS
KW  -  RAT AORTA
KW  -  EXPRESSION
KW  -  RECEPTOR
SP  - 1163 
VL  - 161
IS  - 6
SN  - 0021-9525
UR  - https://discovery.ucl.ac.uk/id/eprint/16410/
JF  - J CELL BIOL
A1  - Gerhardt, H
A1  - Golding, M
A1  - Fruttiger, M
A1  - Ruhrberg, C
A1  - Lundkvist, A
A1  - Abramsson, A
A1  - Jeltsch, M
A1  - Mitchell, C
A1  - Alitalo, K
A1  - Shima, D
A1  - Betsholtz, C
ER  -