eprintid: 1571766 rev_number: 39 eprint_status: archive userid: 608 dir: disk0/01/57/17/66 datestamp: 2017-08-30 11:58:39 lastmod: 2021-10-06 22:53:13 status_changed: 2017-08-30 17:44:45 type: article metadata_visibility: show creators_name: Nishimura, T creators_name: Mizushima, N title: The ULK complex initiates autophagosome formation at phosphatidylinositol synthase-enriched ER subdomains ispublished: pub divisions: UCL divisions: B02 divisions: C08 keywords: ATG9A, RB1CC1, ULK complex, autophagy, phosphatidylinositol synthase note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions. abstract: In our recent paper, we biochemically analyzed autophagosome-related membranes at the initiation stage of macroautophagy/autophagy using atg knockout (KO) cells and demonstrated that the ULK complex is recruited to 2 distinct membranes: the ER membrane and ATG9A-positive autophagosome precursors. We have also identified phosphatidylinositol synthase (PIS)-enriched ER subdomains as the initiation site of autophagosome formation. Based on these findings, we propose that the ULK complex, the PIS-enriched ER subdomain, and ATG9A vesicles together initiate autophagosome formation. date: 2017 date_type: published official_url: http://dx.doi.org/10.1080/15548627.2017.1358344 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1415408 doi: 10.1080/15548627.2017.1358344 lyricists_name: Nishimura, Taki lyricists_id: TNISH35 actors_name: Nishimura, Taki actors_id: TNISH35 actors_role: owner full_text_status: public publication: Autophagy volume: 13 number: 10 pagerange: 1795-1796 event_location: United States issn: 1554-8635 citation: Nishimura, T; Mizushima, N; (2017) The ULK complex initiates autophagosome formation at phosphatidylinositol synthase-enriched ER subdomains. Autophagy , 13 (10) pp. 1795-1796. 10.1080/15548627.2017.1358344 <https://doi.org/10.1080/15548627.2017.1358344>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1571766/1/Nishimura_ULK_complex_initiates.pdf