@article{discovery1571766, note = {This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.}, volume = {13}, number = {10}, journal = {Autophagy}, year = {2017}, title = {The ULK complex initiates autophagosome formation at phosphatidylinositol synthase-enriched ER subdomains}, pages = {1795--1796}, keywords = {ATG9A, RB1CC1, ULK complex, autophagy, phosphatidylinositol synthase}, url = {http://dx.doi.org/10.1080/15548627.2017.1358344}, issn = {1554-8635}, abstract = {In our recent paper, we biochemically analyzed autophagosome-related membranes at the initiation stage of macroautophagy/autophagy using atg knockout (KO) cells and demonstrated that the ULK complex is recruited to 2 distinct membranes: the ER membrane and ATG9A-positive autophagosome precursors. We have also identified phosphatidylinositol synthase (PIS)-enriched ER subdomains as the initiation site of autophagosome formation. Based on these findings, we propose that the ULK complex, the PIS-enriched ER subdomain, and ATG9A vesicles together initiate autophagosome formation.}, author = {Nishimura, T and Mizushima, N} }