eprintid: 1563877 rev_number: 31 eprint_status: archive userid: 608 dir: disk0/01/56/38/77 datestamp: 2017-07-19 11:28:47 lastmod: 2021-09-26 22:18:11 status_changed: 2018-01-24 10:54:53 type: article metadata_visibility: show creators_name: Taslimi, Y creators_name: Sadeghipour, P creators_name: Habibzadeh, S creators_name: Mashayekhi, V creators_name: Mortazavi, H creators_name: Müller, I creators_name: Lane, ME creators_name: Kropf, P creators_name: Rafati, S title: A novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D10 divisions: G08 note: © 2017 Taslimi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. abstract: Accurate diagnosis of cutaneous leishmaniasis (CL) is important for chemotherapy and epidemiological studies. Common approaches for Leishmania detection involve the invasive collection of specimens for direct identification of amastigotes by microscopy and the culturing of promastigotes from infected tissues. Although these techniques are highly specific, they require highly skilled health workers and have the inherent risks of all invasive procedures, such as pain and risk of bacterial and fungal super-infection. Therefore, it is essential to reduce discomfort, potential infection and scarring caused by invasive diagnostic approaches especially for children. In this report, we present a novel non-invasive method, that is painless, rapid and user-friendly, using sequential tape strips for sampling and isolation of DNA from the surface of active and healed skin lesions of CL patients. A total of 119 patients suspected of suffering from cutaneous leishmaniasis with different clinical manifestations were recruited and samples were collected both from their lesions and from uninfected areas. In addition, 15 fungal-infected lesions and 54 areas of healthy skin were examined. The duration of sampling is short (less than one minute) and species identification by PCR is highly specific and sensitive. The sequential tape stripping sampling method is a sensitive, non-invasive and cost-effective alternative to traditional diagnostic assays and it is suitable for field studies as well as for use in health care centers. date: 2017-07-13 date_type: published official_url: https://doi.org/10.1371/journal.pntd.0005750 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1386190 doi: 10.1371/journal.pntd.0005750 pii: PNTD-D-17-00256 lyricists_name: Lane, Majella lyricists_id: MLANE69 actors_name: Lane, Majella actors_id: MLANE69 actors_role: owner full_text_status: public publication: PLoS Neglected Tropical Diseases volume: 11 number: 7 article_number: e0005750 event_location: United States issn: 1935-2735 citation: Taslimi, Y; Sadeghipour, P; Habibzadeh, S; Mashayekhi, V; Mortazavi, H; Müller, I; Lane, ME; ... Rafati, S; + view all <#> Taslimi, Y; Sadeghipour, P; Habibzadeh, S; Mashayekhi, V; Mortazavi, H; Müller, I; Lane, ME; Kropf, P; Rafati, S; - view fewer <#> (2017) A novel non-invasive diagnostic sampling technique for cutaneous leishmaniasis. PLoS Neglected Tropical Diseases , 11 (7) , Article e0005750. 10.1371/journal.pntd.0005750 <https://doi.org/10.1371/journal.pntd.0005750>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1563877/1/Lane_journal.pntd.0005750.pdf