eprintid: 1556760
rev_number: 28
eprint_status: archive
userid: 608
dir: disk0/01/55/67/60
datestamp: 2017-05-20 22:33:02
lastmod: 2021-09-25 23:04:54
status_changed: 2017-09-29 13:13:30
type: article
metadata_visibility: show
creators_name: Chapman, CAR
creators_name: Wang, L
creators_name: Chen, H
creators_name: Garrison, J
creators_name: Lein, PJ
creators_name: Seker, E
title: Nanoporous Gold Biointerfaces: Modifying Nanostructure to Control Neural Cell Coverage and Enhance Electrophysiological Recording Performance
ispublished: pub
divisions: UCL
divisions: B04
divisions: C05
keywords: cell–material interactions; electrophysiology; nanoporous gold; nanostructure libraries; neural interfaces
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
abstract: Nanostructured neural interface coatings have significantly enhanced recording fidelity in both implantable and in vitro devices. As such, nanoporous gold (np-Au) has shown promise as a multifunctional neural interface coating due, in part, to its ability to promote nanostructure-mediated reduction in astrocytic surface coverage while not affecting neuronal coverage. The goal of this study is to provide insight into the mechanisms by which the np-Au nanostructure drives the differential response of neurons versus astrocytes in an in vitro model. Utilizing microfabricated libraries that display varying feature sizes of np-Au, it is demonstrated that np-Au influences neural cell coverage through modulating focal adhesion formation in a feature size-dependent manner. The results here show that surfaces with small (≈30 nm) features control astrocyte spreading through inhibition of focal adhesion formation, while surfaces with large (≈170 nm and greater) features control astrocyte spreading through other mechanotransduction mechanisms. This cellular response combined with lower electrical impedance of np-Au electrodes significantly enhances the fidelity and stability of electrophysiological recordings from cortical neuron-glia co-cultures relative to smooth gold electrodes. Finally, by leveraging the effect of nanostructure on neuronal versus glial cell attachment, the use of laser-based nanostructure modulation is demonstrated for selectively patterning neurons with micrometer spatial resolution.
date: 2017-01-19
date_type: published
official_url: http://doi.org/10.1002/adfm.201604631
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1294495
doi: 10.1002/adfm.201604631
lyricists_name: Chapman, Christopher
lyricists_id: ARCHA48
full_text_status: public
publication: Advanced Functional Materials
volume: 27
number: 3
article_number: 1604631
issn: 1616-301X
citation:        Chapman, CAR;    Wang, L;    Chen, H;    Garrison, J;    Lein, PJ;    Seker, E;      (2017)    Nanoporous Gold Biointerfaces: Modifying Nanostructure to Control Neural Cell Coverage and Enhance Electrophysiological Recording Performance.                   Advanced Functional Materials , 27  (3)    , Article 1604631.  10.1002/adfm.201604631 <https://doi.org/10.1002/adfm.201604631>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1556760/1/Chapman_Nanoporous%20Gold%20Biointerfaces.pdf