eprintid: 1554592
rev_number: 30
eprint_status: archive
userid: 608
dir: disk0/01/55/45/92
datestamp: 2017-04-30 04:27:02
lastmod: 2021-09-20 00:01:43
status_changed: 2017-06-12 14:56:44
type: article
metadata_visibility: show
creators_name: Pina, MF
creators_name: Lau, W
creators_name: Scherer, K
creators_name: Parhizkar, M
creators_name: Edirisinghe, M
creators_name: Craig, D
title: The generation of compartmentalized nanoparticles containing siRNA and cisplatin using a multi-needle electrohydrodynamic strategy
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D10
divisions: G08
divisions: B04
divisions: C05
divisions: F45
keywords: Science & Technology, Physical Sciences, Technology, Chemistry, Multidisciplinary, Nanoscience & Nanotechnology, Materials Science, Multidisciplinary, Physics, Applied, Chemistry, Science & Technology - Other Topics, Materials Science, Physics, MESOPOROUS SILICA NANOPARTICLES, OVERCOME DRUG-RESISTANCE, CANCER-CELLS, CO-DELIVERY, FLUORESCENCE MICROSCOPY, ANTICANCER DRUG, IN-VIVO, CHEMOTHERAPY, COMBINATION, DOXORUBICIN
note: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
abstract: This study outlines a novel manufacturing technique for the generation of compartmentalized trilayered nanoparticles loaded with an anti-cancer agent and siRNA as a platform for the combination treatment of cancers. More specifically, we describe the use of a multi-needle electrohydrodynamic approach to produce nanoparticles with high size specificity and scalable output, while allowing suitable environments for each therapeutic agent. The inner polylactic-glycolic-acid (PLGA) layer was loaded with cisplatin while the middle chitosan layer was loaded with siRNA. The corresponding polymeric solutions were characterized for their viscosity, surface tension and conductivity, while particle size was determined using dynamic light scattering. The internal structure was studied using transmission electron microscopy (TEM) and Structured Illumination Microscopy (SIM). The inclusion of cisplatin was studied using electron dispersive spectroscopy (EDS). We were able to generate nanoparticles of approximate size 130 nm with three distinct layers containing an outer protective PLGA layer, a middle layer of siRNA and an inner layer of cisplatin. These particles have the potential not only for uptake into tumors via the enhanced permeability and retention (EPR) effect but also the sequential release of the siRNA and chemotherapeutic agent, thereby providing a means of overcoming challenges of targeting and tumor drug resistance.
date: 2017-05-14
date_type: published
publisher: ROYAL SOC CHEMISTRY
official_url: http://doi.org/10.1039/c7nr01002h
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: Article
verified: verified_manual
elements_id: 1291321
doi: 10.1039/c7nr01002h
lyricists_name: Craig, Duncan
lyricists_name: Edirisinghe, Mohan
lyricists_name: Lau, Wai
lyricists_name: Parhizkar, Maryam
lyricists_id: DQMCR05
lyricists_id: MJEDE48
lyricists_id: WKLAU81
lyricists_id: MPARH34
actors_name: Craig, Duncan
actors_name: Laslett, David
actors_id: DQMCR05
actors_id: DLASL34
actors_role: owner
actors_role: impersonator
full_text_status: public
publication: Nanoscale
volume: 9
number: 18
pagerange: 5975-5985
pages: 11
issn: 2040-3372
citation:        Pina, MF;    Lau, W;    Scherer, K;    Parhizkar, M;    Edirisinghe, M;    Craig, D;      (2017)    The generation of compartmentalized nanoparticles containing siRNA and cisplatin using a multi-needle electrohydrodynamic strategy.                   Nanoscale , 9  (18)   pp. 5975-5985.    10.1039/c7nr01002h <https://doi.org/10.1039/c7nr01002h>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1554592/1/Craig_c7nr01002h.pdf