eprintid: 1554592 rev_number: 30 eprint_status: archive userid: 608 dir: disk0/01/55/45/92 datestamp: 2017-04-30 04:27:02 lastmod: 2021-09-20 00:01:43 status_changed: 2017-06-12 14:56:44 type: article metadata_visibility: show creators_name: Pina, MF creators_name: Lau, W creators_name: Scherer, K creators_name: Parhizkar, M creators_name: Edirisinghe, M creators_name: Craig, D title: The generation of compartmentalized nanoparticles containing siRNA and cisplatin using a multi-needle electrohydrodynamic strategy ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D10 divisions: G08 divisions: B04 divisions: C05 divisions: F45 keywords: Science & Technology, Physical Sciences, Technology, Chemistry, Multidisciplinary, Nanoscience & Nanotechnology, Materials Science, Multidisciplinary, Physics, Applied, Chemistry, Science & Technology - Other Topics, Materials Science, Physics, MESOPOROUS SILICA NANOPARTICLES, OVERCOME DRUG-RESISTANCE, CANCER-CELLS, CO-DELIVERY, FLUORESCENCE MICROSCOPY, ANTICANCER DRUG, IN-VIVO, CHEMOTHERAPY, COMBINATION, DOXORUBICIN note: This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. abstract: This study outlines a novel manufacturing technique for the generation of compartmentalized trilayered nanoparticles loaded with an anti-cancer agent and siRNA as a platform for the combination treatment of cancers. More specifically, we describe the use of a multi-needle electrohydrodynamic approach to produce nanoparticles with high size specificity and scalable output, while allowing suitable environments for each therapeutic agent. The inner polylactic-glycolic-acid (PLGA) layer was loaded with cisplatin while the middle chitosan layer was loaded with siRNA. The corresponding polymeric solutions were characterized for their viscosity, surface tension and conductivity, while particle size was determined using dynamic light scattering. The internal structure was studied using transmission electron microscopy (TEM) and Structured Illumination Microscopy (SIM). The inclusion of cisplatin was studied using electron dispersive spectroscopy (EDS). We were able to generate nanoparticles of approximate size 130 nm with three distinct layers containing an outer protective PLGA layer, a middle layer of siRNA and an inner layer of cisplatin. These particles have the potential not only for uptake into tumors via the enhanced permeability and retention (EPR) effect but also the sequential release of the siRNA and chemotherapeutic agent, thereby providing a means of overcoming challenges of targeting and tumor drug resistance. date: 2017-05-14 date_type: published publisher: ROYAL SOC CHEMISTRY official_url: http://doi.org/10.1039/c7nr01002h oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green article_type_text: Article verified: verified_manual elements_id: 1291321 doi: 10.1039/c7nr01002h lyricists_name: Craig, Duncan lyricists_name: Edirisinghe, Mohan lyricists_name: Lau, Wai lyricists_name: Parhizkar, Maryam lyricists_id: DQMCR05 lyricists_id: MJEDE48 lyricists_id: WKLAU81 lyricists_id: MPARH34 actors_name: Craig, Duncan actors_name: Laslett, David actors_id: DQMCR05 actors_id: DLASL34 actors_role: owner actors_role: impersonator full_text_status: public publication: Nanoscale volume: 9 number: 18 pagerange: 5975-5985 pages: 11 issn: 2040-3372 citation: Pina, MF; Lau, W; Scherer, K; Parhizkar, M; Edirisinghe, M; Craig, D; (2017) The generation of compartmentalized nanoparticles containing siRNA and cisplatin using a multi-needle electrohydrodynamic strategy. Nanoscale , 9 (18) pp. 5975-5985. 10.1039/c7nr01002h <https://doi.org/10.1039/c7nr01002h>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1554592/1/Craig_c7nr01002h.pdf