TY  - JOUR
SN  - 1932-6203
UR  - http://doi.org/10.1371/journal.pone.0174447
ID  - discovery1552825
N2  - Background:  
Stromal derived factor-1? (SDF-1?/CXCL12) is a chemokine that is up-regulated in diseases
characterised by tissue hypoxia, including myocardial infarction, ischaemic cardiomyopathy
and remote ischaemic conditioning (RIC), a technique of cyclical, non-injurious
ischaemia applied remote from the heart that protects the heat from lethal ischaemia-reperfusion
injury. Accordingly, there is considerable interest in SDF-1? as a potential biomarker
of such conditions. However, SDF-1? is rapidly degraded and inactivated by dipeptidyl peptidase
4 and other peptidases, and the kinetics of intact SDF-1? remain unknown.
Methods & results:  
To facilitate investigation of full-length SDF-1? we established an ELISA using a novel
recombinant human antibody we developed called HCI.SDF1. HCI.SDF1 is specific to the
N-terminal sequence of all isoforms of SDF-1 and has a comparable KD to commercially
available antibodies. Together with a detection antibody specific to the ?-isoform, HCI.SDF1
was used to specifically quantify full-length SDF-1? in blood for the first time. Using RIC
applied to the hind limb of Sprague-Dawley rats or the arms of healthy human volunteers,
we demonstrate an increase in SDF-1? using a commercially available antibody, as previously
reported, but an unexpected decrease in full-length SDF-1? after RIC in both species.
Conclusions:  
We report for the first time the development of a novel recombinant antibody specific to fulllength
SDF-1. Applied to RIC, we demonstrate a significant decrease in SDF-1? that is at
odds with the literature and suggests a need to investigate the kinetics of full-length SDF-1?
in conditions characterised by tissue hypoxia.
KW  - Enzyme-linked immunoassays
KW  -  Blood plasma
KW  -  Platelets
KW  -  Library screening
KW  -  Myocardial infarction
KW  -  Ischemia
KW  -  Peptide libraries
KW  -  Biomarkers
A1  - Bromage, DI
A1  - Taferner, S
A1  - Pillai, M
A1  - Yellon, DM
A1  - Davidson, SM
JF  - PLoS One
Y1  - 2017///
AV  - public
VL  - 12
TI  - A novel recombinant antibody specific to full-length stromal derived factor-1 for potential application in biomarker studies
N1  - Copyright: © 2017 Bromage et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
IS  - 4
ER  -