TY  - UNPB
PB  - UCL (University College London)
UR  - https://discovery.ucl.ac.uk/id/eprint/1547646/
ID  - discovery1547646
N2  - The assessment of skin permeation is critically important in many fields
and a suitable and robust model that may be used to quantify and predict
percutaneous penetration is necessary. Currently available models include in
vitro models using human skin, animal skin, synthetic membranes and cell
culture models. Recently, the Skin Parallel Artificial Membrane Permeation
Assay (PAMPA) has been proposed as a simple but high throughput screening
system that may be suitable to study skin permeation.
In the present study, a lipophilic active, ibuprofen and a hydrophilic active,
caffeine were selected to conduct in vitro permeation studies in the
conventional Franz cell models using silicone membrane, porcine skin and
human skin, and the novel Skin PAMPA model. The overarching aim was to
determine the utility of Skin PAMPA for routine in vitro skin permeation testing
with reference to topical formulations.
The in vitro permeation studies conducted in Franz cell models using
silicone membrane and porcine skin showed that as a lipophilic active,
ibuprofen permeated rapidly though silicone membrane and porcine skin.
Compared with ibuprofen, caffeine went through the skin more slowly. In vitro
permeation studies in Franz cell models using human skin indicated that the
ibuprofen percentage permeation values for human skin were much lower than
corresponding values in porcine skin as expected. 
Various in vitro permeation studies were conducted in the novel skin
PAMPA model for different ibuprofen and caffeine formulations. In general, the
Skin PAMPA model did discriminate between different formulation types and
different solvent systems compared with other models, with low variability in
the permeation data. The more permeable nature of the PAMPA, silicone
membrane and porcine tissue models to ibuprofen compared with human skin
was also demonstrated, while the permeation of caffeine, a hydrophilic
compound, in the PAMPA model was comparable to that in human skin.
A1  - Luo, L
M1  - Doctoral
EP  - 267
Y1  - 2017/04/28/
AV  - public
TI  - Mechanistic studies on skin permeation models
N1  - Unpublished
ER  -