eprintid: 1546859 rev_number: 24 eprint_status: archive userid: 608 dir: disk0/01/54/68/59 datestamp: 2017-04-01 19:34:46 lastmod: 2020-02-12 21:45:17 status_changed: 2017-09-06 13:50:22 type: article metadata_visibility: show creators_name: Pickering, H creators_name: Burr, SE creators_name: Derrick, T creators_name: Makalo, P creators_name: Joof, H creators_name: Hayward, RD creators_name: Holland, MJ title: Profiling and validation of individual and patterns of Chlamydia trachomatis-specific antibody responses in trachomatous trichiasis ispublished: pub divisions: UCL divisions: A01 divisions: B02 divisions: C08 keywords: Antibodies, Chlamydia trachomatis, Microarray, Trachoma, Trachomatous scarring, Trachomatous trichiasis note: Copyright © The Author(s) 2017. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. abstract: BACKGROUND: Ocular Chlamydia trachomatis (Ct) infection causes trachoma, the leading infectious cause of blindness. A Ct D/UW3 proteome microarray and sera from Gambian adults with trachomatous trichiasis (TT) or healthy matched controls previously identified several novel antigens, which suggested differential recognition in adults with TT. METHODS: We re-analysed this serological microarray data using more robust microarray analysis techniques accounting for typical problems associated with highly dimensional data. We examined the Ct-specific antibody profile concerning the overall diversity of responses, antigen expression stage and cellular localisation of antigens. We tested differentially recognised antigens by further serological testing of the screened sera and used larger independent sample sets for validation. RESULTS: Antibody responses identified High-Performance on antigens expressed early and late in the Ct developmental cycle and those secreted or localised to the outer membrane. Eight antigens were preferentially recognised by scarred individuals and one antigen by healthy individuals. Three of these antigens, two associated with scarring (CT667 and CT706) and one healthy-associated (CT442), were not associated with the presence or absence of scarring following specific serological testing of the arrayed sera and sera from larger, independent case-control cohorts. CONCLUSIONS: This study identified focussed Ct-specific antibody profiles targeting proteins expressed during entry and exit from cells and localised to interact with the host. A small panel of antibody responses could discriminate between adults with and without TT in a trachoma-endemic community. Heterogenous responses in the independent validation of these antibody targets highlighted the need for large sample sizes, clearly defined clinical phenotypes and follow-up work. date: 2017-03-13 date_type: published official_url: http://dx.doi.org/10.1186/s13071-017-2078-8 oa_status: green full_text_type: pub pmcid: PMC5347170 language: eng primo: open primo_central: open_green verified: verified_manual elements_id: 1267880 doi: 10.1186/s13071-017-2078-8 pii: 10.1186/s13071-017-2078-8 lyricists_name: Hayward, Richard lyricists_id: RDHAY42 actors_name: Allington-Smith, Dominic actors_id: DAALL44 actors_role: owner full_text_status: public publication: Parasites & Vectors volume: 10 number: 1 article_number: 143 event_location: England issn: 1756-3305 citation: Pickering, H; Burr, SE; Derrick, T; Makalo, P; Joof, H; Hayward, RD; Holland, MJ; (2017) Profiling and validation of individual and patterns of Chlamydia trachomatis-specific antibody responses in trachomatous trichiasis. Parasites & Vectors , 10 (1) , Article 143. 10.1186/s13071-017-2078-8 <https://doi.org/10.1186/s13071-017-2078-8>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1546859/1/Profiling%20and%20validation%20of%20individual%20and%20patterns%20of%20Chlamydia%20trachomatis-specific%20antibody%20responses%20in%20trachomatous%20trichiasis.pdf