eprintid: 1544269
rev_number: 24
eprint_status: archive
userid: 608
dir: disk0/01/54/42/69
datestamp: 2017-05-05 13:36:24
lastmod: 2021-10-02 22:03:04
status_changed: 2017-05-05 13:36:24
type: thesis
metadata_visibility: show
creators_name: Woollard, ACS
title: Neuromuscular reinnervation efficacy after nerve repair or nerve graft in YFP mice
ispublished: unpub
divisions: UCL
divisions: B02
divisions: C10
divisions: D16
abstract: INTRODUCTION: The gold standard reconstruction for facial reanimation is the functional muscle transfer. The reinnervation of a muscle is never complete and clinical results are variable with 20% not achieving a satisfactory outcome (1). This study uses transgenic YFP mice to investigate the patterns of reinnervation through a nerve repair and a nerve graft. METHOD: 81 YFP-16 mice were studied in three intervention groups (nerve repair, nerve graft and nerve cut) over three time periods (four, six and twelve weeks post intervention). Two parameters were investigated: the number and surface area of reinnervated neuromuscular junctions and the number and calibre of regenerating axons. An assessment was made of motor unit proportions. 14 YFP-H mice were used to further investigate the patterns of axon regeneration through a nerve repair and graft. RESULTS: In the nerve cut group there was no reinnervation of the muscle at any time period. In all cases of nerve repair and nerve graft the muscles were completely reinnervated by regenerating axons with no significant difference between either intervention group and controls. The number and calibre of the regenerating axons was significantly different to controls for both intervention groups. The motor units were smaller in both intervention groups, but when the axons were thresholded to within two standard deviations of the control axon populations, the motor units were larger. DISCUSSION: Reinnervation after nerve repair or graft appeared adequate, however the neuromuscular junctions showed some evidence of morphological change that persisted. The number and type of axons after repair or graft differed from the control groups. There were more small calibre axons, suggesting the arbor had been reinnervated by a smaller number of axons that branched to increase their influence. These axons showed evidence of remodeling in the repair group, but not in the graft group.
date: 2017-03-28
oa_status: green
full_text_type: other
thesis_class: doctoral_open
language: eng
thesis_view: UCL_Thesis
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1212836
lyricists_name: Grobblelaar, Adriaan Ockert
lyricists_name: Woollard, Alexander
lyricists_id: AOGRO05
lyricists_id: ACSWO25
actors_name: Woollard, Alexander
actors_name: Laslett, David
actors_id: ACSWO25
actors_id: DLASL34
actors_role: owner
actors_role: impersonator
full_text_status: public
pages: 219
event_title: UCL (University College London)
institution: UCL (University College London)
department: Surgery and Intervention Sciences
thesis_type: Doctoral
citation:        Woollard, ACS;      (2017)    Neuromuscular reinnervation efficacy after nerve repair or nerve graft in YFP mice.                   Doctoral thesis , UCL (University College London).     Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1544269/1/Woollard_PhD%20Final%202017%20edited.pdf