eprintid: 1540375
rev_number: 34
eprint_status: archive
userid: 608
dir: disk0/01/54/03/75
datestamp: 2017-02-16 14:34:28
lastmod: 2021-09-17 22:08:39
status_changed: 2017-02-16 14:34:28
type: article
metadata_visibility: show
creators_name: Griffin, M
creators_name: Ryan, CM
creators_name: Pathan, O
creators_name: Abraham, D
creators_name: Denton, CP
creators_name: Butler, PE
title: Characteristics of human adipose derived stem cells in scleroderma in comparison to sex and age matched normal controls: implications for regenerative medicine.
ispublished: pub
divisions: UCL
divisions: B02
divisions: C10
divisions: D17
divisions: G90
divisions: D16
keywords: Adipose-derived stem cells, Invasion, Metabolism, Migration, Proliferation, Regenerative medicine, Scleroderma, Systemic sclerosis
note: © The Author(s). 2017
Open Access
This article is distributed under the terms of the Creative Commons Attribution 4.0
International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and
reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to
the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver
(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
abstract: BACKGROUND: Adipose-derived stem cells (ADSCs) are emerging as an alternative stem cell source for cell-based therapies. Recent data suggest that autologous ADSC-enriched micrografting improves the effects of facial involvement in systemic sclerosis (SSc). We have extensively characterised ADSCs from SSc patients and compared their phenotype and function to healthy age- and sex-matched control ADSCs. METHODS: ADSCs were isolated and characterised from a cohort of six SSc patients (ADSC-SSc) and were compared to six healthy age- and sex-matched controls (ADSC-N). Cell surface phenotype lineage commitment was explored by flow cytometric analysis of mesenchymal and hematopoietic markers and by the capacity to differentiate to chondrogenic, osteogenic, and adipogenic lineages. Functional activities of ADSCs were assessed by biochemical and cellular assays for proliferation, metabolism, adhesion, morphology, migration, and invasion. RESULTS: Upon characterization of ADSC-SSc, we found that there was no alteration in the phenotype or surface antigen expression compared to healthy matched control ADSCs. We found that the differentiation capacity of ADSC-SSc was equivalent to that of ADSC-N, and that ADSC-SSc did not display any morphological or adhesive abnormalities. We found that the proliferation rate and metabolic activity of ADSC-SSc was reduced (p < 0.01). We found that the migration and invasion capacity of ADSC-SSc was reduced (p < 0.01) compared to healthy matched control ADSCs. CONCLUSIONS: This study provides important findings that can differentially characterise ADSCs from SSc patients. Results indicate that the surface phenotype and differentiation capacity of ADSCs from SSc patients are identical to healthy matched ADSCs. While the findings indicate that the proliferation and migration capacity of ADSC-SSc is reduced, ADSC-SSc are capable of ex-vivo culture and expansion. These findings encourage further investigation into the understanding by which ADSCs can impact upon tissue fibrosis.
date: 2017-02-07
date_type: published
official_url: https://doi.org/10.1186/s13287-016-0444-7
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: Journal Article
verified: verified_manual
elements_id: 1207574
doi: 10.1186/s13287-016-0444-7
pii: 10.1186/s13287-016-0444-7
language_elements: eng
lyricists_name: Abraham, David
lyricists_name: Denton, Christopher
lyricists_name: Griffin, Michelle
lyricists_id: DJABR26
lyricists_id: CPDEN87
lyricists_id: GRFFI78
actors_name: Zahnhausen-Stuber, Petra
actors_id: PMZAH20
actors_role: owner
full_text_status: public
publication: Stem Cell Res Ther
volume: 8
number: 1
article_number: 23
event_location: England
issn: 1757-6512
citation:        Griffin, M;    Ryan, CM;    Pathan, O;    Abraham, D;    Denton, CP;    Butler, PE;      (2017)    Characteristics of human adipose derived stem cells in scleroderma in comparison to sex and age matched normal controls: implications for regenerative medicine.                   Stem Cell Res Ther , 8  (1)    , Article 23.  10.1186/s13287-016-0444-7 <https://doi.org/10.1186/s13287-016-0444-7>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1540375/7/Denton_art_10.1186_s13287-016-0444-7.pdf