eprintid: 1538337
rev_number: 24
eprint_status: archive
userid: 608
dir: disk0/01/53/83/37
datestamp: 2017-01-28 22:05:50
lastmod: 2021-10-10 22:54:52
status_changed: 2017-04-11 11:17:37
type: article
metadata_visibility: show
creators_name: Sanfilippo, C
creators_name: Nunnari, G
creators_name: Calcagno, A
creators_name: Malaguarnera, L
creators_name: Blennow, K
creators_name: Zetterberg, H
creators_name: Di Rosa, M
title: The chitinases expression is related to Simian Immunodeficiency Virus Encephalitis (SIVE) and in HIV encephalitis (HIVE)
ispublished: pub
divisions: UCL
divisions: B02
divisions: C07
divisions: D07
divisions: F86
keywords: Chitinases, HIV-1, HIVE, Hippocampus, SIVE
note: This version is the author accepted manuscript. For information on re-use, please refer to the publisher’s terms and conditions.
abstract: OBJECTIVES: Human Immunodeficiency Virus (HIV) infection can induce neurocognitive complications classified as HIV-associated neurocognitive disorder (HAND). The chitinase family is associated with innate immunity cells and many infectious diseases. METHODS: We analyzed microarray datasets obtained from NCBI in order to verify the expression of chitinase family genes in hippocampus of uninfected rhesus macaques versus those with histopathologic evidence of Simian Immunodeficiency Virus Encephalitis (SIVE). Moreover, we have analysed two human microarray datasets to verify the results obtained in macaques hippocampus affected by SIVE. For these studies, we have also used the open source tools Genome-scale Integrated Analysis of gene Networks in Tissues (GIANT) to identify the chitinase genes network. RESULTS: CHIT1, CHI3L1 and CHI3L2 levels were significantly increased in SIVE hippocampus as compared to non-infected control specimens. Furthermore, we found a negative correlation between CHIA vs. Brain Viral Load (BVL). These data was confirmed partially in human brain section of HAD/HIVE subjects. Also, we showed that HIV-1 was able to modulate the expression of CHIT1, CHI3L1, CHI3L2 and CHID1 in human macrophages. CONCLUSIONS: These results suggest that chitinase gene expression is altered in SIVE and in HAD/HIVE brain sections and call for more studies examining whether this is a protective immunological reaction or a destructive tissue response to encephalitis.
date: 2017-01-02
date_type: published
official_url: http://doi.org/10.1016/j.virusres.2016.10.012
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
article_type_text: Journal Article
verified: verified_manual
elements_id: 1189956
doi: 10.1016/j.virusres.2016.10.012
pii: S0168-1702(16)30395-1
lyricists_name: Zetterberg, Henrik
lyricists_id: HZETT94
actors_name: Zetterberg, Henrik
actors_name: Laslett, David
actors_id: HZETT94
actors_id: DLASL34
actors_role: owner
actors_role: impersonator
full_text_status: public
publication: Virus Research
volume: 227
pagerange: 220-230
event_location: Netherlands
issn: 1872-7492
citation:        Sanfilippo, C;    Nunnari, G;    Calcagno, A;    Malaguarnera, L;    Blennow, K;    Zetterberg, H;    Di Rosa, M;      (2017)    The chitinases expression is related to Simian Immunodeficiency Virus Encephalitis (SIVE) and in HIV encephalitis (HIVE).                   Virus Research , 227    pp. 220-230.    10.1016/j.virusres.2016.10.012 <https://doi.org/10.1016/j.virusres.2016.10.012>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1538337/1/Zetterberg_SanfilippoCHIT.pdf