eprintid: 1528546
rev_number: 26
eprint_status: archive
userid: 608
dir: disk0/01/52/85/46
datestamp: 2016-11-13 01:52:31
lastmod: 2021-11-23 01:14:18
status_changed: 2016-12-07 18:33:12
type: article
metadata_visibility: show
creators_name: Jean, R-D
creators_name: Larsson, M
creators_name: Cheng, W-D
creators_name: Hsu, Y-Y
creators_name: Bow, J-S
creators_name: Liu, D-M
title: Design and optimization of a nanoprobe comprising amphiphilic chitosan colloids and Au-nanorods: Sensitive detection of human serum albumin in simulated urine
ispublished: pub
divisions: UCL
divisions: B04
divisions: C05
keywords: Science & Technology, Physical Sciences, Technology, Chemistry, Physical, Materials Science, Coatings & Films, Physics, Applied, Physics, Condensed Matter, Chemistry, Materials Science, Physics, Surface plasmon resonance biosensor, Chitosan nanoparticles, Gold nanorods, Human serum albumin, Simulated urine, Gold Nanorods, Dna Hybridization, Nanoparticles, Therapy, Biosensors, Spectra, Probes, Size
note: Copyright © 2016 Elsevier B.V. All rights reserved. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
abstract: Metallic nanoparticles have been utilized as analytical tools to detect a wide range of organic analytes. In most reports, gold (Au)-based nanosensors have been modified with ligands to introduce selectivity towards a specific target molecule. However, in a recent study a new concept was presented where bare Au-nanorods on self-assembled carboxymethyl-hexanoyl chitosan (CHC) nanocarriers achieved sensitive and selective detection of human serum albumin (HSA) after manipulation of the solution pH. Here this concept was further advanced through optimization of the ratio between Au-nanorods and CHC nanocarriers to create a nanotechnology-based sensor (termed CHC-AuNR nanoprobe) with an outstanding lower detection limit (LDL) for HSA. The CHC-AuNR nanoprobe was evaluated in simulated urine solution and a LDL as low as 1.5 pM was achieved at an estimated AuNR/CHC ratio of 2. Elemental mapping and protein adsorption kinetics over three orders of magnitude in HSA concentration confirmed accumulation of HSA on the nanorods and revealed the adsorption to be completed within 15 min for all investigated concentrations. The results suggest that the CHC-AuNR nanoprobe has potential to be utilized for cost-effective detection of analytes in complex liquids.
date: 2016-12-30
date_type: published
publisher: ELSEVIER SCIENCE BV
official_url: http://dx.doi.org/10.1016/j.apsusc.2016.08.143
oa_status: green
full_text_type: other
language: eng
primo: open
primo_central: open_green
article_type_text: Article
verified: verified_manual
elements_id: 1191015
doi: 10.1016/j.apsusc.2016.08.143
lyricists_name: Larsson, Lars
lyricists_id: LMLAR62
full_text_status: public
publication: Applied Surface Science
volume: 390
pagerange: 675-680
pages: 6
issn: 0169-4332
citation:        Jean, R-D;    Larsson, M;    Cheng, W-D;    Hsu, Y-Y;    Bow, J-S;    Liu, D-M;      (2016)    Design and optimization of a nanoprobe comprising amphiphilic chitosan colloids and Au-nanorods: Sensitive detection of human serum albumin in simulated urine.                   Applied Surface Science , 390    pp. 675-680.    10.1016/j.apsusc.2016.08.143 <https://doi.org/10.1016/j.apsusc.2016.08.143>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1528546/1/Jean_Design%20and%20optimization%20of%20a%20nanoprobe%20comprising%20amphiphilic%20chitosan%20colloids%20and%20Au-nanorods.pdf