eprintid: 1527523 rev_number: 30 eprint_status: archive userid: 608 dir: disk0/01/52/75/23 datestamp: 2016-11-10 14:24:41 lastmod: 2021-09-26 22:33:26 status_changed: 2016-11-10 14:24:41 type: article metadata_visibility: show creators_name: Wang, SX creators_name: Acha, D creators_name: Shah, AJ creators_name: Hills, F creators_name: Roitt, I creators_name: Demosthenous, A creators_name: Bayford, RH title: Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor ispublished: pub divisions: UCL divisions: B04 divisions: C05 divisions: F46 keywords: Alzheimer's disease; Biomarker; Electrochemical biosensor; Impedance; Tau note: Copyright © 2016. This manuscript version is published under a Creative Commons Attribution Non-commercial Non-derivative 4.0 International licence (CC BY-NC-ND 4.0). This licence allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licences are available at http://creativecommons.org/licenses/by/4.0. Access may be initially restricted by the publisher. abstract: This study presents a novel approach based on a four-electrode electrochemical biosensor for the detection of tau protein – one of the possible markers for the prediction of Alzheimer's disease (AD). The biosensor is based on the formation of stable antibody–antigen complexes on gold microband electrodes covered with a layer of a self-assembled monolayer and protein G. Antibodies were immobilized on the gold electrode surface in an optimal orientation by protein G interaction. Electrochemical impedance spectroscopy was used to analyze impedance change, which revealed a linear response with increasing tau concentrations. The assay is fast (<1 h for incubation and measurement) and very sensitive. The limit of quantification for the full-length 2N4R tau protein is 0.03 pM, a value unaltered when the assay was processed in bovine serum albumin or human serum. This technology could be adapted for the detection of other biomarkers to provide a multiple assay to identify AD progression in a point of care setting. date: 2017-06-15 date_type: published official_url: http://dx.doi.org/10.1016/j.bios.2016.10.077 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green article_type_text: Article verified: verified_manual elements_id: 1190250 doi: 10.1016/j.bios.2016.10.077 lyricists_name: Bayford, Richard lyricists_name: Demosthenous, Andreas lyricists_id: RBAYF91 lyricists_id: ACDEM08 actors_name: Demosthenous, Andreas actors_id: ACDEM08 actors_role: owner full_text_status: public publication: Biosensors and Bioelectronics volume: 92 pagerange: 482-488 issn: 0956-5663 citation: Wang, SX; Acha, D; Shah, AJ; Hills, F; Roitt, I; Demosthenous, A; Bayford, RH; (2017) Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor. Biosensors and Bioelectronics , 92 pp. 482-488. 10.1016/j.bios.2016.10.077 <https://doi.org/10.1016/j.bios.2016.10.077>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1527523/1/Detection%20of%20Tau%20Biosensor.pdf