TY  - JOUR
A1  - Wang, SX
A1  - Acha, D
A1  - Shah, AJ
A1  - Hills, F
A1  - Roitt, I
A1  - Demosthenous, A
A1  - Bayford, RH
KW  - Alzheimer's disease; Biomarker; Electrochemical biosensor; Impedance; Tau
JF  - Biosensors and Bioelectronics
UR  - http://dx.doi.org/10.1016/j.bios.2016.10.077
SN  - 0956-5663
N2  - This study presents a novel approach based on a four-electrode electrochemical biosensor for the detection of tau protein ? one of the possible markers for the prediction of Alzheimer's disease (AD). The biosensor is based on the formation of stable antibody?antigen complexes on gold microband electrodes covered with a layer of a self-assembled monolayer and protein G. Antibodies were immobilized on the gold electrode surface in an optimal orientation by protein G interaction. Electrochemical impedance spectroscopy was used to analyze impedance change, which revealed a linear response with increasing tau concentrations. The assay is fast (<1 h for incubation and measurement) and very sensitive. The limit of quantification for the full-length 2N4R tau protein is 0.03 pM, a value unaltered when the assay was processed in bovine serum albumin or human serum. This technology could be adapted for the detection of other biomarkers to provide a multiple assay to identify AD progression in a point of care setting.
ID  - discovery1527523
N1  - Copyright © 2016. This manuscript version is published under a Creative Commons Attribution Non-commercial Non-derivative 4.0 International licence (CC BY-NC-ND 4.0). This licence allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licences are available at http://creativecommons.org/licenses/by/4.0. Access may be initially restricted by the publisher.
AV  - public
SP  - 482
VL  - 92
Y1  - 2017/06/15/
EP  - 488
TI  - Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor
ER  -