%0 Journal Article
%@ 0956-5663
%A Wang, SX
%A Acha, D
%A Shah, AJ
%A Hills, F
%A Roitt, I
%A Demosthenous, A
%A Bayford, RH
%D 2017
%F discovery:1527523
%J Biosensors and Bioelectronics
%K Alzheimer's disease; Biomarker; Electrochemical biosensor; Impedance; Tau
%P 482-488
%T Detection of the tau protein in human serum by a sensitive four-electrode electrochemical biosensor
%U https://discovery.ucl.ac.uk/id/eprint/1527523/
%V 92
%X This study presents a novel approach based on a four-electrode electrochemical biosensor for the detection of tau protein – one of the possible markers for the prediction of Alzheimer's disease (AD). The biosensor is based on the formation of stable antibody–antigen complexes on gold microband electrodes covered with a layer of a self-assembled monolayer and protein G. Antibodies were immobilized on the gold electrode surface in an optimal orientation by protein G interaction. Electrochemical impedance spectroscopy was used to analyze impedance change, which revealed a linear response with increasing tau concentrations. The assay is fast (<1 h for incubation and measurement) and very sensitive. The limit of quantification for the full-length 2N4R tau protein is 0.03 pM, a value unaltered when the assay was processed in bovine serum albumin or human serum. This technology could be adapted for the detection of other biomarkers to provide a multiple assay to identify AD progression in a point of care setting.
%Z Copyright © 2016. This manuscript version is published under a Creative Commons Attribution Non-commercial Non-derivative 4.0 International licence (CC BY-NC-ND 4.0). This licence allows you to share, copy, distribute and transmit the work for personal and non-commercial use providing author and publisher attribution is clearly stated. Further details about CC BY licences are available at http://creativecommons.org/licenses/by/4.0. Access may be initially restricted by the publisher.