eprintid: 1516783
rev_number: 26
eprint_status: archive
userid: 608
dir: disk0/01/51/67/83
datestamp: 2016-09-24 23:13:51
lastmod: 2021-09-27 22:26:32
status_changed: 2017-07-11 16:19:51
type: article
metadata_visibility: show
creators_name: Juen, MA
creators_name: Wunderlich, CH
creators_name: Nussbaumer, F
creators_name: Tollinger, M
creators_name: Kontaxis, G
creators_name: Konrat, R
creators_name: Hansen, DF
creators_name: Kreutz, C
title: Excited States of Nucleic Acids Probed by Proton Relaxation Dispersion NMR Spectroscopy
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D09
divisions: G03
keywords: Science & Technology, Physical Sciences, Chemistry, Multidisciplinary, Chemistry, DNA, NMR spectroscopy, proton relaxation dispersion, RNA, stable isotope labeling, HIV-1 NUCLEOCAPSID PROTEIN, H-1-NMR CHEMICAL-SHIFTS, RNA DYNAMICS, CTAR DNA, Conformational States, H-1, Time, Nucleotides, Recognition, Chaperone
note: © 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
abstract: In this work an improved stable isotope labeling protocol for nucleic acids is introduced. The novel building blocks eliminate/minimize homonuclear 13C and 1H scalar couplings thus allowing proton relaxation dispersion (RD) experiments to report accurately on the chemical exchange of nucleic acids. Using site-specific 2H and 13C labeling, spin topologies are introduced into DNA and RNA that make 1H relaxation dispersion experiments applicable in a straightforward manner. The novel RNA/DNA building blocks were successfully incorporated into two nucleic acids. The A-site RNA was previously shown to undergo a two site exchange process in the micro- to millisecond time regime. Using proton relaxation dispersion experiments the exchange parameters determined earlier could be recapitulated, thus validating the proposed approach. We further investigated the dynamics of the cTAR DNA, a DNA transcript that is involved in the viral replication cycle of HIV-1. Again, an exchange process could be characterized and quantified. This shows the general applicablility of the novel labeling scheme for 1H RD experiments of nucleic acids.
date: 2016-08-17
date_type: published
publisher: WILEY-V C H VERLAG GMBH
official_url: http://dx.doi.org/10.1002/anie.201605870
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: Article
verified: verified_manual
elements_id: 1155028
doi: 10.1002/anie.201605870
language_elements: eng
lyricists_name: Hansen, Dennis Flemming
lyricists_id: DFHAN88
full_text_status: public
publication: Angewandte Chemie International Edition
volume: 55
number: 39
pagerange: 12008-12012
pages: 5
issn: 1433-7851
citation:        Juen, MA;    Wunderlich, CH;    Nussbaumer, F;    Tollinger, M;    Kontaxis, G;    Konrat, R;    Hansen, DF;           Juen, MA;  Wunderlich, CH;  Nussbaumer, F;  Tollinger, M;  Kontaxis, G;  Konrat, R;  Hansen, DF;  Kreutz, C;   - view fewer <#>    (2016)    Excited States of Nucleic Acids Probed by Proton Relaxation Dispersion NMR Spectroscopy.                   Angewandte Chemie International Edition , 55  (39)   pp. 12008-12012.    10.1002/anie.201605870 <https://doi.org/10.1002/anie.201605870>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1516783/1/Juen_et_al-2016-Angewandte_Chemie.pdf