eprintid: 1516783 rev_number: 26 eprint_status: archive userid: 608 dir: disk0/01/51/67/83 datestamp: 2016-09-24 23:13:51 lastmod: 2021-09-27 22:26:32 status_changed: 2017-07-11 16:19:51 type: article metadata_visibility: show creators_name: Juen, MA creators_name: Wunderlich, CH creators_name: Nussbaumer, F creators_name: Tollinger, M creators_name: Kontaxis, G creators_name: Konrat, R creators_name: Hansen, DF creators_name: Kreutz, C title: Excited States of Nucleic Acids Probed by Proton Relaxation Dispersion NMR Spectroscopy ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D09 divisions: G03 keywords: Science & Technology, Physical Sciences, Chemistry, Multidisciplinary, Chemistry, DNA, NMR spectroscopy, proton relaxation dispersion, RNA, stable isotope labeling, HIV-1 NUCLEOCAPSID PROTEIN, H-1-NMR CHEMICAL-SHIFTS, RNA DYNAMICS, CTAR DNA, Conformational States, H-1, Time, Nucleotides, Recognition, Chaperone note: © 2016 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. abstract: In this work an improved stable isotope labeling protocol for nucleic acids is introduced. The novel building blocks eliminate/minimize homonuclear 13C and 1H scalar couplings thus allowing proton relaxation dispersion (RD) experiments to report accurately on the chemical exchange of nucleic acids. Using site-specific 2H and 13C labeling, spin topologies are introduced into DNA and RNA that make 1H relaxation dispersion experiments applicable in a straightforward manner. The novel RNA/DNA building blocks were successfully incorporated into two nucleic acids. The A-site RNA was previously shown to undergo a two site exchange process in the micro- to millisecond time regime. Using proton relaxation dispersion experiments the exchange parameters determined earlier could be recapitulated, thus validating the proposed approach. We further investigated the dynamics of the cTAR DNA, a DNA transcript that is involved in the viral replication cycle of HIV-1. Again, an exchange process could be characterized and quantified. This shows the general applicablility of the novel labeling scheme for 1H RD experiments of nucleic acids. date: 2016-08-17 date_type: published publisher: WILEY-V C H VERLAG GMBH official_url: http://dx.doi.org/10.1002/anie.201605870 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green article_type_text: Article verified: verified_manual elements_id: 1155028 doi: 10.1002/anie.201605870 language_elements: eng lyricists_name: Hansen, Dennis Flemming lyricists_id: DFHAN88 full_text_status: public publication: Angewandte Chemie International Edition volume: 55 number: 39 pagerange: 12008-12012 pages: 5 issn: 1433-7851 citation: Juen, MA; Wunderlich, CH; Nussbaumer, F; Tollinger, M; Kontaxis, G; Konrat, R; Hansen, DF; Juen, MA; Wunderlich, CH; Nussbaumer, F; Tollinger, M; Kontaxis, G; Konrat, R; Hansen, DF; Kreutz, C; - view fewer <#> (2016) Excited States of Nucleic Acids Probed by Proton Relaxation Dispersion NMR Spectroscopy. Angewandte Chemie International Edition , 55 (39) pp. 12008-12012. 10.1002/anie.201605870 <https://doi.org/10.1002/anie.201605870>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1516783/1/Juen_et_al-2016-Angewandte_Chemie.pdf