eprintid: 1516779
rev_number: 28
eprint_status: archive
userid: 608
dir: disk0/01/51/67/79
datestamp: 2016-09-24 23:11:28
lastmod: 2021-11-25 23:31:39
status_changed: 2017-07-26 15:05:53
type: article
metadata_visibility: show
creators_name: Choudhary, P
creators_name: Whiting, PJ
title: A strategy to ensure safety of stem cell-derived retinal pigment epithelium cells
ispublished: pub
divisions: UCL
divisions: B02
divisions: C07
divisions: D07
divisions: F85
keywords: Retinal pigment epithelium, Embryonic stem cells, Flow sorting, Age-related macular degeneration, Cell surface markers
note: Copyright © 2016 The Author(s). Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and
reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver
(http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
abstract: Cell replacement and regenerative therapy using embryonic stem cell-derived material holds promise for the treatment of several pathologies. However, the safety of this approach is of prime importance given the teratogenic potential of residual stem cells, if present in the differentiated cell product. Using the example of embryonic stem cell-derived retinal pigment epithelium (RPE) for the treatment of age-related macular degeneration, we present a novel strategy for ensuring the absence of stem cells in the RPE population. Based on an unbiased screening approach, we identify and validate the expression of CD59, a cell surface marker expressed on RPE but absent on stem cells. We further demonstrate that flow sorting on the basis of CD59 expression can effectively purify RPE and deplete stem cells, resulting in a population free from stem cell impurity. This purification helps to ensure removal of stem cells and hence increases the safety of cells that may be used for clinical transplantation. This strategy can potentially be applied to other pluripotent stem cell-derived material and help mitigate concerns of using such cells for therapy.
date: 2016-09-02
date_type: published
publisher: BIOMED CENTRAL LTD
official_url: http://dx.doi.org/10.1186/s13287-016-0380-6
oa_status: green
full_text_type: pub
pmcid: PMC5010679
language: eng
primo: open
primo_central: open_green
article_type_text: Article
verified: verified_manual
elements_id: 1157821
doi: 10.1186/s13287-016-0380-6
lyricists_name: Whiting, Paul
lyricists_id: PWHIT17
actors_name: Flynn, Bernadette
actors_id: BFFLY94
actors_role: owner
full_text_status: public
publication: Stem Cell Research & Therapy
volume: 7
article_number: 127
pages: 8
issn: 1757-6512
citation:        Choudhary, P;    Whiting, PJ;      (2016)    A strategy to ensure safety of stem cell-derived retinal pigment epithelium cells.                   Stem Cell Research & Therapy , 7     , Article 127.  10.1186/s13287-016-0380-6 <https://doi.org/10.1186/s13287-016-0380-6>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1516779/1/Choudhary_Strategy_ensure_safety.pdf