eprintid: 1508432 rev_number: 18 eprint_status: archive userid: 608 dir: disk0/01/50/84/32 datestamp: 2016-08-22 13:06:25 lastmod: 2020-02-12 22:02:37 status_changed: 2016-08-22 13:06:25 type: article metadata_visibility: show creators_name: Jiang, ZH creators_name: Chen, QY creators_name: Harrison, TJ creators_name: Li, GJ creators_name: Wang, XY creators_name: Li, H creators_name: Hu, LP creators_name: Li, KW creators_name: Yang, QL creators_name: Tan, C creators_name: Fang, ZL title: Hepatitis B Virus Core Promoter Double Mutations (A1762T, G1764A) Are Associated with Lower Levels of Serum Dihydrolipoyl Dehydrogenase ispublished: pub divisions: UCL divisions: A01 divisions: B02 divisions: C10 divisions: D17 note: © 2016 The Author(s). This article is licensed under the Creative Commons Attribution 4.0 International License (CC BY). Usage, derivative works and distribution are permitted provided that proper credit is given to the author and the original publisher. abstract: Published by S. Karger AG, BaselObjectives: The aim of this study was to identify serum proteins with differential concentrations between hepatocellular carcinoma (HCC) patients and HBsAg asymptomatic carriers among individuals infected with hepatitis B virus (HBV) with basal core promoter (BCP) double mutations (A1762T, G1764A). Methods: iTRAQ and liquid chromatography-tandem mass spectrometry were used to identify differentially expressed protein, and an ELISA test was used for the validation test. Results: The total number of proteins identified was 1,125, of which 239 showed statistically significant differences in their expression. The relative concentrations of serum dihydrolipoyl dehydrogenase (DLD), which showed the most significant correlation with liver diseases and infection, were significantly lower in HCC patients than asymptomatic HBsAg carriers and individuals negative for HBsAg. However, only the difference between HCC patients with BCP double mutations and HBsAg-negative individuals could be confirmed by ELISA. Meanwhile, we found that the concentrations of serum DLD in those infected with HBV with BCP double mutations were significantly lower than in individuals with the wild-type BCP. However, the difference in the concentrations of serum DLD between individuals with wild-type BCP and those negative for HBsAg was not significant. Conclusions: HBV with BCP double mutations are associated with lower concentrations of serum DLD. date: 2016-06-16 date_type: published official_url: http://dx.doi.org/10.1159/000445319 oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green article_type_text: Article in Press verified: verified_manual elements_id: 1140554 doi: 10.1159/000445319 lyricists_name: Harrison, Tim lyricists_id: THARR65 actors_name: Harrison, Tim actors_name: Barczynska, Patrycja actors_id: THARR65 actors_id: PBARC91 actors_role: owner actors_role: impersonator full_text_status: public publication: Intervirology volume: 59 number: 1 pagerange: 1-7 issn: 1423-0100 citation: Jiang, ZH; Chen, QY; Harrison, TJ; Li, GJ; Wang, XY; Li, H; Hu, LP; ... Fang, ZL; + view all <#> Jiang, ZH; Chen, QY; Harrison, TJ; Li, GJ; Wang, XY; Li, H; Hu, LP; Li, KW; Yang, QL; Tan, C; Fang, ZL; - view fewer <#> (2016) Hepatitis B Virus Core Promoter Double Mutations (A1762T, G1764A) Are Associated with Lower Levels of Serum Dihydrolipoyl Dehydrogenase. Intervirology , 59 (1) pp. 1-7. 10.1159/000445319 <https://doi.org/10.1159/000445319>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1508432/1/document%281%29.pdf