eprintid: 1494973
rev_number: 27
eprint_status: archive
userid: 608
dir: disk0/01/49/49/73
datestamp: 2016-05-29 10:54:35
lastmod: 2021-09-26 22:30:14
status_changed: 2017-01-09 16:36:56
type: article
metadata_visibility: show
creators_name: Howe, K
creators_name: Schiffer, PH
creators_name: Zielinski, J
creators_name: Wiehe, T
creators_name: Laird, GK
creators_name: Marioni, JC
creators_name: Soylemez, O
creators_name: Kondrashov, F
creators_name: Leptin, M
title: Structure and evolutionary history of a large family of NLR proteins in the zebrafish
ispublished: pub
divisions: UCL
divisions: B16
divisions: B14
divisions: J79
note: © 2016 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.
abstract: Multicellular eukaryotes have evolved a range of mechanisms for immune recognition. A widespread family involved in innate immunity are the NACHT-domain and leucine-rich-repeat-containing (NLR) proteins. Mammals have small numbers of NLR proteins, whereas in some species, mostly those without adaptive immune systems, NLRs have expanded into very large families. We describe a family of nearly 400 NLR proteins encoded in the zebrafish genome. The proteins share a defining overall structure, which arose in fishes after a fusion of the core NLR domains with a B30.2 domain, but can be subdivided into four groups based on their NACHT domains. Gene conversion acting differentially on the NACHT and B30.2 domains has shaped the family and created the groups. Evidence of positive selection in the B30.2 domain indicates that this domain rather than the leucine-rich repeats acts as the pathogen recognition module. In an unusual chromosomal organization, the majority of the genes are located on one chromosome arm, interspersed with other large multigene families, including a new family encoding zinc-finger proteins. The NLR-B30.2 proteins represent a new family with diversity in the specific recognition module that is present in fishes in spite of the parallel existence of an adaptive immune system.
date: 2016-04
date_type: published
official_url: http://dx.doi.org/10.1098/rsob.160009
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: Article
verified: verified_manual
elements_id: 1129919
doi: 10.1098/rsob.160009
lyricists_name: Schiffer, Philipp
lyricists_id: PSCHI65
actors_name: Bracey, Alan
actors_id: ABBRA90
actors_role: owner
full_text_status: public
publication: Open Biology
volume: 6
number: 4
article_number: 160009
issn: 2046-2441
citation:        Howe, K;    Schiffer, PH;    Zielinski, J;    Wiehe, T;    Laird, GK;    Marioni, JC;    Soylemez, O;         ... Leptin, M; + view all <#>        Howe, K;  Schiffer, PH;  Zielinski, J;  Wiehe, T;  Laird, GK;  Marioni, JC;  Soylemez, O;  Kondrashov, F;  Leptin, M;   - view fewer <#>    (2016)    Structure and evolutionary history of a large family of NLR proteins in the zebrafish.                   Open Biology , 6  (4)    , Article 160009.  10.1098/rsob.160009 <https://doi.org/10.1098/rsob.160009>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1494973/1/160009.full.pdf