eprintid: 1493075
rev_number: 35
eprint_status: archive
userid: 608
dir: disk0/01/49/30/75
datestamp: 2016-06-16 13:47:16
lastmod: 2021-09-26 22:59:20
status_changed: 2016-06-16 13:47:16
type: article
metadata_visibility: show
creators_name: Twelvetrees, AE
creators_name: Pernigo, S
creators_name: Sanger, A
creators_name: Guedes-Dias, P
creators_name: Schiavo, G
creators_name: Steiner, RA
creators_name: Dodding, MP
creators_name: Holzbaur, EL
title: The Dynamic Localization of Cytoplasmic Dynein in Neurons Is Driven by Kinesin-1
ispublished: pub
divisions: UCL
divisions: B02
divisions: C07
divisions: D07
divisions: F85
note: Copyright © 2016 The Authors. Published by Elsevier Inc.
This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
abstract: Cytoplasmic dynein, the major motor driving retrograde axonal transport, must be actively localized to axon terminals. This localization is critical as dynein powers essential retrograde trafficking events required for neuronal survival, such as neurotrophic signaling. Here, we demonstrate that the outward transport of dynein from soma to axon terminal is driven by direct interactions with the anterograde motor kinesin-1. In developing neurons, we find that dynein dynamically cycles between neurites, following kinesin-1 and accumulating in the nascent axon coincident with axon specification. In established axons, dynein is constantly transported down the axon at slow axonal transport speeds; inhibition of the kinesin-1-dynein interaction effectively blocks this process. In vitro and live-imaging assays to investigate the underlying mechanism lead us to propose a new model for the slow axonal transport of cytosolic cargos, based on short-lived direct interactions of cargo with a highly processive anterograde motor.
date: 2016-06-01
date_type: published
official_url: http://dx.doi.org/10.1016/j.neuron.2016.04.046
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_id: 1130494
doi: 10.1016/j.neuron.2016.04.046
pii: S0896-6273(16)30160-X
lyricists_name: Schiavo, Giampietro
lyricists_name: Twelvetrees, Alison
lyricists_id: GSCHI71
lyricists_id: AETWE75
actors_name: Twelvetrees, Alison
actors_id: AETWE75
actors_role: owner
full_text_status: public
publication: Neuron
volume: 90
number: 5
pagerange: 1000-1015
issn: 1097-4199
citation:        Twelvetrees, AE;    Pernigo, S;    Sanger, A;    Guedes-Dias, P;    Schiavo, G;    Steiner, RA;    Dodding, MP;           Twelvetrees, AE;  Pernigo, S;  Sanger, A;  Guedes-Dias, P;  Schiavo, G;  Steiner, RA;  Dodding, MP;  Holzbaur, EL;   - view fewer <#>    (2016)    The Dynamic Localization of Cytoplasmic Dynein in Neurons Is Driven by Kinesin-1.                   Neuron , 90  (5)   pp. 1000-1015.    10.1016/j.neuron.2016.04.046 <https://doi.org/10.1016/j.neuron.2016.04.046>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1493075/1/Twelvetrees%20et%20al%202016%20Dynamic%20localization.pdf