eprintid: 1485960 rev_number: 48 eprint_status: archive userid: 608 dir: disk0/01/48/59/60 datestamp: 2016-04-30 23:05:58 lastmod: 2021-11-10 01:46:40 status_changed: 2016-06-30 10:35:04 type: article metadata_visibility: show creators_name: Konstantinou, G creators_name: Kirkby, PA creators_name: Evans, GJ creators_name: Naga Srinivas Nadella, KM creators_name: Griffiths, VA creators_name: Mitchell, JE creators_name: Angus Silver, R title: Dynamic wavefront shaping with an acousto-optic lens for laser scanning microscopy ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D09 divisions: G02 divisions: B04 divisions: C05 divisions: F46 note: Copyright © 2016 Optical Society of America. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited abstract: Acousto-optic deflectors (AODs) arranged in series and driven with linearly chirped frequencies can rapidly focus and tilt optical wavefronts, enabling high-speed 3D random access microscopy. Non-linearly chirped acoustic drive frequencies can also be used to shape the optical wavefront allowing a range of higher-order aberrations to be generated. However, to date, wavefront shaping with AODs has been achieved by using single laser pulses for strobed illumination to 'freeze' the moving acoustic wavefront, limiting voxel acquisition rates. Here we show that dynamic wavefront shaping can be achieved by applying non-linear drive frequencies to a pair of AODs with counter-propagating acoustic waves, which comprise a cylindrical acousto-optic lens (AOL). Using a cylindrical AOL we demonstrate high-speed continuous axial line scanning and the first experimental AOL-based correction of a cylindrical lens aberration at 30 kHz, accurate to 1/35<sup>th</sup> of a wave at 800 nm. Furthermore, we develop a model to show how spherical aberration, which is the major aberration in AOL-based remote-focusing systems, can be partially or fully corrected with AOLs consisting of four or six AODs, respectively. date: 2016-03-21 date_type: published official_url: http://dx.doi.org/10.1364/OE.24.006283 oa_status: green full_text_type: other language: eng primo: open primo_central: open_green article_type_text: Journal Article verified: verified_manual elements_id: 1127272 doi: 10.1364/OE.24.006283 pii: 338139 lyricists_name: Griffiths, Victoria lyricists_name: Kirkby, Paul lyricists_name: Konstantinou, George lyricists_name: Mitchell, John lyricists_name: Nadella, K M Naga Srinivas lyricists_name: Silver, Robin lyricists_id: VAGRI23 lyricists_id: PAKIR56 lyricists_id: GKONS76 lyricists_id: JMITC40 lyricists_id: KMNSN48 lyricists_id: ASILV65 full_text_status: public publication: Optics Express volume: 24 number: 6 pagerange: 6283-6299 event_location: United States issn: 1094-4087 citation: Konstantinou, G; Kirkby, PA; Evans, GJ; Naga Srinivas Nadella, KM; Griffiths, VA; Mitchell, JE; Angus Silver, R; (2016) Dynamic wavefront shaping with an acousto-optic lens for laser scanning microscopy. Optics Express , 24 (6) pp. 6283-6299. 10.1364/OE.24.006283 <https://doi.org/10.1364/OE.24.006283>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1485960/7/Mitchell_Dynamic%20wavefront%20shaping.pdf