TY  - JOUR
IS  - 1
N1  - © 2016 Authors; published by Portland Press Limited.
VL  - 44
SP  - 286
JF  - Biochemical Society Transactions
A1  - Cockcroft, S
A1  - Garner, K
A1  - Yadav, S
A1  - Gomez-Espinoza, E
A1  - Raghu, P
UR  - http://dx.doi.org/10.1042/BST20150228
SN  - 1470-8752
TI  - RdgB? reciprocally transfers PA and PI at ER-PM contact sites to maintain PI(4,5)P2 homoeostasis during phospholipase C signalling in Drosophila photoreceptors
AV  - public
Y1  - 2016/02/15/
EP  - 292
KW  - 5-bisphosphate [PI(4
KW  - 5)P2]
KW  -  endoplasmic reticulum?plasma membrane (ER?PM) contact sites
KW  -  phosphatidic acid
KW  -  phosphatidic acid (PA) transport proteins
KW  -  phosphatidylinositol
KW  -  phosphatidylinositol 4
KW  -  phosphatidylinositol transfer protein (PITP)
KW  -  phospholipase C
KW  -  phototransduction
KW  -  retinal degeneration type B (RdgB)
N2  - Phosphatidylinositol (PI) is the precursor lipid for the synthesis of PI 4,5-bisphosphate [PI(4,5)P2] at the plasma membrane (PM) and is sequentially phosphorylated by the lipid kinases, PI 4-kinase and phosphatidylinositol 4-phosphate (PI4P)-5-kinase. Receptor-mediated hydrolysis of PI(4,5)P2 takes place at the PM but PI resynthesis occurs at the endoplasmic reticulum (ER). Thus PI(4,5)P2 resynthesis requires the reciprocal transport of two key intermediates, phosphatidic acid (PA) and PI between the ER and the PM. PI transfer proteins (PITPs), defined by the presence of the PITP domain, can facilitate lipid transfer between membranes; the PITP domain comprises a hydrophobic cavity with dual specificity but accommodates a single phospholipid molecule. The class II PITP, retinal degeneration type B (RdgB)? is a multi-domain protein and its PITP domain can bind and transfer PI and PA. In Drosophila photoreceptors, a well-defined G-protein-coupled phospholipase C? (PLC?) signalling pathway, phototransduction defects resulting from loss of RdgB? can be rescued by expression of the PITP domain provided it is competent for both PI and PA transfer. We propose that RdgB? proteins maintain PI(4,5)P2 homoeostasis after PLC activation by facilitating the reciprocal transport of PA and PI at ER-PM membrane contact sites.
ID  - discovery1473392
ER  -