eprintid: 1472713 rev_number: 22 eprint_status: archive userid: 608 dir: disk0/01/47/27/13 datestamp: 2015-11-19 16:05:12 lastmod: 2021-09-19 23:27:40 status_changed: 2015-11-19 16:05:12 type: article metadata_visibility: show creators_name: Le Duigou, C creators_name: Savary, E creators_name: Kullmann, DM creators_name: Miles, R title: Induction of Anti-Hebbian LTP in CA1 Stratum Oriens Interneurons: Interactions between Group I Metabotropic Glutamate Receptors and M1 Muscarinic Receptors ispublished: pub divisions: UCL divisions: B02 divisions: C07 divisions: D07 divisions: F81 keywords: LTP, hippocampus, interneuron, metabotropic glutamate receptors, muscarinic receptors note: Copyright © 2015 Le Duigou et al. This is an Open Access article distributed under the terms of the Creative Commons Attribution License Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed abstract: An anti-Hebbian form of LTP is observed at excitatory synapses made with some hippocampal interneurons. LTP induction is facilitated when postsynaptic interneurons are hyperpolarized, presumably because Ca(2+) entry through Ca(2+)-permeable glutamate receptors is enhanced. The contribution of modulatory transmitters to anti-Hebbian LTP induction remains to be established. Activation of group I metabotropic receptors (mGluRs) is required for anti-Hebbian LTP induction in interneurons with cell bodies in the CA1 stratum oriens. This region receives a strong cholinergic innervation from the septum, and muscarinic acetylcholine receptors (mAChRs) share some signaling pathways and cooperate with mGluRs in the control of neuronal excitability.We therefore examined possible interactions between group I mGluRs and mAChRs in anti-Hebbian LTP at synapses which excite oriens interneurons in rat brain slices. We found that blockade of either group I mGluRs or M1 mAChRs prevented the induction of anti-Hebbian LTP by pairing presynaptic activity with postsynaptic hyperpolarization. Blocking either receptor also suppressed long-term effects of activation of the other G-protein coupled receptor on interneuron membrane potential. However, no crossed blockade was detected for mGluR or mAchR effects on interneuron after-burst potentials or on the frequency of miniature EPSPs. Paired recordings between pyramidal neurons and oriens interneurons were obtained to determine whether LTP could be induced without concurrent stimulation of cholinergic axons. Exogenous activation of mAChRs led to LTP, with changes in EPSP amplitude distributions consistent with a presynaptic locus of expression. LTP, however, required noninvasive presynaptic and postsynaptic recordings. date: 2015-10-07 date_type: published official_url: http:/d/x.doi.org/10.1523/JNEUROSCI.0956-15.2015 oa_status: green full_text_type: pub pmcid: PMC4595614 language: eng primo: open primo_central: open_green article_type_text: Journal Article verified: verified_manual elements_id: 1067770 doi: 10.1523/JNEUROSCI.0956-15.2015 pii: 35/40/13542 language_elements: eng lyricists_name: Kullmann, Dimitri lyricists_id: DMKUL63 actors_name: Kullmann, Dimitri actors_name: Barczynska, Patrycja actors_id: DMKUL63 actors_id: PBARC91 actors_role: owner actors_role: impersonator full_text_status: public publication: Journal of Neuroscience volume: 35 number: 40 pagerange: 13542-13554 event_location: United States issn: 1529-2401 citation: Le Duigou, C; Savary, E; Kullmann, DM; Miles, R; (2015) Induction of Anti-Hebbian LTP in CA1 Stratum Oriens Interneurons: Interactions between Group I Metabotropic Glutamate Receptors and M1 Muscarinic Receptors. Journal of Neuroscience , 35 (40) pp. 13542-13554. 10.1523/JNEUROSCI.0956-15.2015 <https://doi.org/10.1523/JNEUROSCI.0956-15.2015>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1472713/1/13542.full.pdf