eprintid: 1472661
rev_number: 22
eprint_status: archive
userid: 608
dir: disk0/01/47/26/61
datestamp: 2015-11-17 14:01:15
lastmod: 2021-11-01 01:36:47
status_changed: 2015-11-17 14:01:15
type: article
metadata_visibility: show
creators_name: Cremona, A
creators_name: Orsini, F
creators_name: Corsetto, PA
creators_name: Hoogenboom, BW
creators_name: Rizzo, AM
title: Reversible Dissolution of Microdomains in Detergent-Resistant Membranes at Physiological Temperature
ispublished: pub
divisions: UCL
divisions: B04
divisions: C06
divisions: F60
note: © 2015 Cremona et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
abstract: The formation of lipid microdomains ("rafts") is presumed to play an important role in various cellular functions, but their nature remains controversial. Here we report on microdomain formation in isolated, detergent-resistant membranes from MDA-MB-231 human breast cancer cells, studied by atomic force microscopy (AFM). Whereas microdomains were readily observed at room temperature, they shrunk in size and mostly disappeared at higher temperatures. This shrinking in microdomain size was accompanied by a gradual reduction of the height difference between the microdomains and the surrounding membrane, consistent with the behaviour expected for lipids that are laterally segregated in liquid ordered and liquid disordered domains. Immunolabeling experiments demonstrated that the microdomains contained flotillin-1, a protein associated with lipid rafts. The microdomains reversibly dissolved and reappeared, respectively, on heating to and cooling below temperatures around 37 °C, which is indicative of radical changes in local membrane order close to physiological temperature.
date: 2015-06-06
date_type: published
official_url: http://dx.doi.org/10.1371/journal.pone.0132696
oa_status: green
full_text_type: pub
pmcid: PMC4493071
language: eng
primo: open
primo_central: open_green
article_type_text: Journal Article
verified: verified_manual
elements_id: 1042380
doi: 10.1371/journal.pone.0132696
pii: PONE-D-15-02638
language_elements: eng
lyricists_name: Hoogenboom, Bart
lyricists_id: BWHOO59
actors_name: Hoogenboom, Bart
actors_name: Barczynska, Patrycja
actors_id: BWHOO59
actors_id: PBARC91
actors_role: owner
actors_role: impersonator
full_text_status: public
publication: PLoS One
volume: 10
number: 7
article_number: e0132696
event_location: United States
issn: 1932-6203
citation:        Cremona, A;    Orsini, F;    Corsetto, PA;    Hoogenboom, BW;    Rizzo, AM;      (2015)    Reversible Dissolution of Microdomains in Detergent-Resistant Membranes at Physiological Temperature.                   PLoS One , 10  (7)    , Article e0132696.  10.1371/journal.pone.0132696 <https://doi.org/10.1371/journal.pone.0132696>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1472661/1/Reversible%20Dissolution%20of%20Microdomains%20in%20Detergent-Resistant%20Membranes%20at%20Physiological%20Temperature..pdf