eprintid: 1472400 rev_number: 22 eprint_status: archive userid: 608 dir: disk0/01/47/24/00 datestamp: 2015-11-09 14:54:09 lastmod: 2021-09-19 23:41:44 status_changed: 2015-11-09 14:54:09 type: article metadata_visibility: show creators_name: Scarpa, E creators_name: Szabó, A creators_name: Bibonne, A creators_name: Theveneau, E creators_name: Parsons, M creators_name: Mayor, R title: Cadherin Switch during EMT in Neural Crest Cells Leads to Contact Inhibition of Locomotion via Repolarization of Forces ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D09 divisions: F96 note: This is an open access article under the CC BY license ( http://creativecommons.org/licenses/by/4.0/) abstract: Contact inhibition of locomotion (CIL) is the process through which cells move away from each other after cell-cell contact, and it contributes to malignant invasion and developmental migration. Various cell types exhibit CIL, whereas others remain in contact after collision and may form stable junctions. To investigate what determines this differential behavior, we study neural crest cells, a migratory stem cell population whose invasiveness has been likened to cancer metastasis. By comparing pre-migratory and migratory neural crest cells, we show that the switch from E- to N-cadherin during EMT is essential for acquisition of CIL behavior. Loss of E-cadherin leads to repolarization of protrusions, via p120 and Rac1, resulting in a redistribution of forces from intercellular tension to cell-matrix adhesions, which break down the cadherin junction. These data provide insight into the balance of physical forces that contributes to CIL in cells in vivo. date: 2015-08-24 date_type: published official_url: http://dx.doi.org/10.1016/j.devcel.2015.06.012 oa_status: green full_text_type: pub pmcid: PMC4552721 primo: open primo_central: open_green article_type_text: Journal Article verified: verified_manual elements_id: 1045384 doi: 10.1016/j.devcel.2015.06.012 pii: S1534-5807(15)00401-3 language_elements: eng lyricists_name: Mayor, Roberto lyricists_name: Szabo, Andras lyricists_id: RMAYO52 lyricists_id: ASZAB39 actors_name: Mayor, Roberto actors_name: Gibson, Alice actors_id: RMAYO52 actors_id: AGIBS47 actors_role: owner actors_role: impersonator full_text_status: public publication: Developmental Cell volume: 34 number: 4 pagerange: 421-434 event_location: United States issn: 1878-1551 citation: Scarpa, E; Szabó, A; Bibonne, A; Theveneau, E; Parsons, M; Mayor, R; (2015) Cadherin Switch during EMT in Neural Crest Cells Leads to Contact Inhibition of Locomotion via Repolarization of Forces. Developmental Cell , 34 (4) pp. 421-434. 10.1016/j.devcel.2015.06.012 <https://doi.org/10.1016/j.devcel.2015.06.012>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1472400/1/1-s2.0-S1534580715004013-main.pdf