eprintid: 1466174
rev_number: 45
eprint_status: archive
userid: 608
dir: disk0/01/46/61/74
datestamp: 2015-04-28 14:26:35
lastmod: 2021-12-16 23:29:33
status_changed: 2015-04-28 14:26:35
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Rizopoulos, Z
creators_name: Balistreri, G
creators_name: Kilcher, S
creators_name: Martin, CK
creators_name: Syedbasha, M
creators_name: Helenius, A
creators_name: Mercer, J
title: Vaccinia Virus Infection Requires Maturation of Macropinosomes.
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
keywords: Endocytosis, Macropinocytosis, PIKfyve, Phosphoinositide exchange, Poxvirus, Rab conversion, Virus entry
note: © 2015 The Authors. Traffic published by John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
abstract: The prototypic poxvirus, vaccinia virus (VACV), occurs in two infectious forms, mature virions (MVs) and extracellular virions (EVs). Both enter HeLa cells by inducing macropinocytic uptake. Using confocal microscopy, live-cell imaging, targeted RNAi screening, and perturbants of endosome maturation, we analyzed the properties and maturation pathway of the macropinocytic vacuoles containing VACV MVs in HeLa cells. The vacuoles first acquired markers of early endosomes [Rab5, EEA1, and phosphatidylinositol(3)P]. Prior to release of virus cores into the cytoplasm, they contained markers of late endosomes and lysosomes (Rab7a, LAMP1, SNX3). RNAi screening of endocytic cell factors emphasized the importance of late compartments for VACV infection. Follow-up perturbation analysis showed that infection required Rab7a and PIKfyve, confirming that VACV is a late penetrating virus dependent on macropinosome maturation. VACV EV infection was inhibited by depletion of many of the same factors, indicating that both infectious particle forms share the need for late vacuolar conditions for penetration.
date: 2015-04-14
official_url: http://dx.doi.org/10.1111/tra.12290
oa_status: green
full_text_type: pub
primo: open
primo_central: open_green
article_type_text: JOURNAL ARTICLE
verified: verified_manual
elements_source: PubMed
elements_id: 1026421
doi: 10.1111/tra.12290
lyricists_name: Mercer, Jason
lyricists_id: JMERC50
full_text_status: public
publication: Traffic
volume: 16
number: 8
pagerange: 814-831
citation:        Rizopoulos, Z;    Balistreri, G;    Kilcher, S;    Martin, CK;    Syedbasha, M;    Helenius, A;    Mercer, J;      (2015)    Vaccinia Virus Infection Requires Maturation of Macropinosomes.                   Traffic , 16  (8)   pp. 814-831.    10.1111/tra.12290 <https://doi.org/10.1111/tra.12290>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1466174/1/tra12290.pdf