eprintid: 1466163
rev_number: 31
eprint_status: archive
userid: 608
dir: disk0/01/46/61/63
datestamp: 2015-04-20 12:50:23
lastmod: 2021-10-04 00:23:28
status_changed: 2015-04-20 12:50:23
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Battigelli, A
creators_name: Russier, J
creators_name: Bianco, A
creators_name: Da Ros, T
creators_name: Prato, M
creators_name: Kostarelos, K
creators_name: Wang, JT-W
creators_name: Al-Jamal, KT
title: Ammonium and guanidinium dendron-carbon nanotubes by amidation and click chemistry and their use for siRNA delivery
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
keywords: Carbon nanotubes; Cationic dendrons; siRNA, RNA delivery;Gene therapy
note: © 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. The copyright line for this article was changed on 16 Sept 2014 after original online publication.
abstract: A series of multi-walled carbon nanotube (MWCNT) conjugates is described, functionalized with different dendrons bearing positive charges at their termini (i.e. ammonium or guanidinium groups). The dendrimeric units are anchored to the nanotube scaffolds using two orthogonal synthetic approaches, amidation and click reactions. The final nanohybrids are characterized by complementary analytical techniques, while their ability to interact with siRNA is investigated by means of agarose gel electrophoresis. The demonstration of the cell uptake capacity, the low cytotoxicity, and the ability of these cationic conjugates to silence cytotoxic genes suggests them to be promising carriers for genetic material. Carbon nanotubes functionalized by amidation or click chemistry with dendrons of different generations possessing ammonium or guanidinium groups are synthesized and complexed to siRNA. The assessment of the cell uptake capacity, the low cytotoxicity, and the ability of these cationic conjugates to silence genes shows them to be promising carriers for genetic material. 
date: 2013-11-11
official_url: http://dx.doi.org/10.1002/smll.201300264
vfaculties: VFLS
oa_status: green
full_text_type: pub
primo: open
primo_central: open_green
verified: verified_manual
elements_source: Scopus
elements_id: 919343
doi: 10.1002/smll.201300264
lyricists_name: Kostarelos, Kostas
lyricists_id: KKOST53
full_text_status: public
publication: Small
volume: 9
number: 21
pagerange: 3610 - 3619
issn: 1613-6810
citation:        Battigelli, A;    Russier, J;    Bianco, A;    Da Ros, T;    Prato, M;    Kostarelos, K;    Wang, JT-W;           Battigelli, A;  Russier, J;  Bianco, A;  Da Ros, T;  Prato, M;  Kostarelos, K;  Wang, JT-W;  Al-Jamal, KT;   - view fewer <#>    (2013)    Ammonium and guanidinium dendron-carbon nanotubes by amidation and click chemistry and their use for siRNA delivery.                   Small , 9  (21)   3610 - 3619.    10.1002/smll.201300264 <https://doi.org/10.1002/smll.201300264>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1466163/1/3610_ftp.pdf