eprintid: 1466163 rev_number: 31 eprint_status: archive userid: 608 dir: disk0/01/46/61/63 datestamp: 2015-04-20 12:50:23 lastmod: 2021-10-04 00:23:28 status_changed: 2015-04-20 12:50:23 type: article metadata_visibility: show item_issues_count: 0 creators_name: Battigelli, A creators_name: Russier, J creators_name: Bianco, A creators_name: Da Ros, T creators_name: Prato, M creators_name: Kostarelos, K creators_name: Wang, JT-W creators_name: Al-Jamal, KT title: Ammonium and guanidinium dendron-carbon nanotubes by amidation and click chemistry and their use for siRNA delivery ispublished: pub divisions: UCL divisions: B02 divisions: C08 keywords: Carbon nanotubes; Cationic dendrons; siRNA, RNA delivery;Gene therapy note: © 2013 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. The copyright line for this article was changed on 16 Sept 2014 after original online publication. abstract: A series of multi-walled carbon nanotube (MWCNT) conjugates is described, functionalized with different dendrons bearing positive charges at their termini (i.e. ammonium or guanidinium groups). The dendrimeric units are anchored to the nanotube scaffolds using two orthogonal synthetic approaches, amidation and click reactions. The final nanohybrids are characterized by complementary analytical techniques, while their ability to interact with siRNA is investigated by means of agarose gel electrophoresis. The demonstration of the cell uptake capacity, the low cytotoxicity, and the ability of these cationic conjugates to silence cytotoxic genes suggests them to be promising carriers for genetic material. Carbon nanotubes functionalized by amidation or click chemistry with dendrons of different generations possessing ammonium or guanidinium groups are synthesized and complexed to siRNA. The assessment of the cell uptake capacity, the low cytotoxicity, and the ability of these cationic conjugates to silence genes shows them to be promising carriers for genetic material. date: 2013-11-11 official_url: http://dx.doi.org/10.1002/smll.201300264 vfaculties: VFLS oa_status: green full_text_type: pub primo: open primo_central: open_green verified: verified_manual elements_source: Scopus elements_id: 919343 doi: 10.1002/smll.201300264 lyricists_name: Kostarelos, Kostas lyricists_id: KKOST53 full_text_status: public publication: Small volume: 9 number: 21 pagerange: 3610 - 3619 issn: 1613-6810 citation: Battigelli, A; Russier, J; Bianco, A; Da Ros, T; Prato, M; Kostarelos, K; Wang, JT-W; Battigelli, A; Russier, J; Bianco, A; Da Ros, T; Prato, M; Kostarelos, K; Wang, JT-W; Al-Jamal, KT; - view fewer <#> (2013) Ammonium and guanidinium dendron-carbon nanotubes by amidation and click chemistry and their use for siRNA delivery. Small , 9 (21) 3610 - 3619. 10.1002/smll.201300264 <https://doi.org/10.1002/smll.201300264>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1466163/1/3610_ftp.pdf