eprintid: 1465969 rev_number: 33 eprint_status: archive userid: 608 dir: disk0/01/46/59/69 datestamp: 2015-04-14 12:21:06 lastmod: 2021-09-20 00:31:08 status_changed: 2015-04-14 12:21:06 type: article metadata_visibility: show item_issues_count: 0 creators_name: Bader, A creators_name: Martini, F creators_name: Schinella, GR creators_name: Rios, JL creators_name: Prieto, JM title: Modulation of Cox-1, 5-, 12- and 15-Lox by popular herbal remedies used in southern Italy against psoriasis and other skin diseases. ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D10 divisions: G09 keywords: Acanthaceae, Asteraceae, herbal medicine, leukotrienes, prostaglandins, psoriasis, Acanthaceae, Achillea, Animals, Anti-Inflammatory Agents, Arachidonate Lipoxygenases, Artemisia, Blood Platelets, Cyclooxygenase 1, Dermatologic Agents, HeLa Cells, Humans, Inula, Italy, Leukocytes, NF-kappa B, Phytotherapy, Plant Extracts, Plants, Medicinal, Psoriasis, Rats, Skin Diseases note: Copyright © 2014 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. abstract: Acanthus mollis (Acanthaceae), Achillea ligustica, Artemisia arborescens and Inula viscosa (Asteraceae) are used in Southern Italy against psoriasis and other skin diseases that occur with an imbalanced production of eicosanoids. We here assessed their in vitro effects upon 5-, 12-, 15-LOX and COX-1 enzymes as well as NFκB activation in intact cells as their possible therapeutic targets. All methanol crude extracts inhibited both 5-LOX and COX-1 activities under 200 µg/mL, without significant effects on the 12-LOX pathway or any relevant in vitro free radical scavenging activity. NFκB activation was prevented by all extracts but A. mollis. Interestingly, A. ligustica, A. arborescens and A. mollis increased the biosynthesis of 15(S)-HETE, an anti-inflammatory eicosanoid. A. ligustica (IC50 =49.5 µg/mL) was superior to Silybum marianum (IC50 =147.8 µg/mL), which we used as antipsoriatic herbal medicine of reference. Its n-hexane, dichloromethane and ethyl acetate fractions had also inhibitory effects on the LTB4 biosynthesis (IC50 s=9.6, 20.3 and 68 µg/mL, respectively) evidencing that the apolar extracts of A. ligustica are promising active herbal ingredients for future phytotherapeutical products targeting psoriasis. date: 2015-01 official_url: http://dx.doi.org/10.1002/ptr.5234 vfaculties: VFLS oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green article_type_text: Journal Article, Research Support, Non-U.S. Gov't verified: verified_manual elements_source: PubMed elements_id: 1010622 doi: 10.1002/ptr.5234 lyricists_name: Prieto Garcia, Jose lyricists_id: JPRIE37 full_text_status: public publication: Phytother Res volume: 29 number: 1 pagerange: 108 - 113 event_location: England citation: Bader, A; Martini, F; Schinella, GR; Rios, JL; Prieto, JM; (2015) Modulation of Cox-1, 5-, 12- and 15-Lox by popular herbal remedies used in southern Italy against psoriasis and other skin diseases. Phytother Res , 29 (1) 108 - 113. 10.1002/ptr.5234 <https://doi.org/10.1002/ptr.5234>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1465969/1/Modulation%20of%20Cox-1%2C%205-%2C%2012-%20and%2015-Lox%20by%20popular%20herbal%20remedies%20used%20in%20southern%20Italy%20against%20psoriasis%20and%20other%20skin%20diseases..pdf