@article{discovery1464075, number = {5}, journal = {Journal of the Optical Society of America B}, pages = {B84-- B98}, title = {Step-by-step guide to the realization of advanced optical tweezers}, year = {2015}, note = {This version is the author accepted manuscript. For information on re-use, please refer to the publisher's terms and conditions.}, volume = {32}, month = {May}, author = {Pesce, Giuseppe and Volpe, Giorgio and Marag{\'o}, Onofrio M and Jones, Philip H and Gigan, Sylvain and Sasso, Antonio and Volpe, Giovanni}, abstract = {Since the pioneering work of Arthur Ashkin, optical tweezers (OT) have become an indispensable tool for contactless manipulation of micro- and nanoparticles. Nowadays OT are employed in a myriad of applications demonstrating their importance. While the basic principle of OT is the use of a strongly focused laser beam to trap and manipulate particles, more complex experimental setups are required to perform novel and challenging experiments. With this article, we provide a detailed step-by-step guide for the construction of advanced optical manipulation systems. First, we explain how to build a single-beam OT on a homemade microscope and how to calibrate it. Improving on this design, we realize a holographic OT, which can manipulate independently multiple particles and generate more sophisticated wavefronts such as Laguerre-Gaussian beams. Finally, we explain how to implement a speckle OT, which permits one to employ random speckle light fields for deterministic optical manipulation.}, url = {http://dx.doi.org/10.1364/JOSAB.32.000B84}, issn = {0740-3224} }