eprintid: 1443051
rev_number: 27
eprint_status: archive
userid: 608
dir: disk0/01/44/30/51
datestamp: 2014-08-25 18:47:26
lastmod: 2021-10-04 00:48:41
status_changed: 2014-08-25 18:47:26
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Tarr, SJ
creators_name: Moon, RW
creators_name: Hardege, I
creators_name: Osborne, AR
title: A conserved domain targets exported PHISTb family proteins to the periphery of Plasmodium infected erythrocytes.
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D09
divisions: G03
keywords: Cytoskeleton, Malaria, PHIST, PRESAN, Plasmodium, Protein export
note: © 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license(http://creativecommons.org/licenses/by/3.0/).
abstract: During blood-stage infection, malaria parasites export numerous proteins to the host erythrocyte. The Poly-Helical Interspersed Sub-Telomeric (PHIST) proteins are an exported family that share a common 'PRESAN' domain, and include numerous members in Plasmodium falciparum, Plasmodium vivax and Plasmodium knowlesi. In P. falciparum, PHIST proteins have been implicated in protein trafficking and intercellular communication. A number of PHIST proteins are essential for parasite survival. Here, we identify nine members of the PHISTb sub-class of PHIST proteins, including one protein known to be essential for parasite survival, that localise to the erythrocyte periphery. These proteins have solubility characteristics consistent with their association with the erythrocyte cytoskeleton. Together, an extended PRESAN domain, comprising the PRESAN domain and preceding sequence, form a novel targeting-domain that is sufficient to localise a protein to the erythrocyte periphery. We validate the role of this domain in RESA, thus identifying a cytoskeleton-binding domain in RESA that functions independently of its known spectrin-binding domain. Our data suggest that some PHISTb proteins may act as cross-linkers of the erythrocyte cytoskeleton. We also show for the first time that peripherally-localised PHISTb proteins are encoded in genomes of P. knowlesi and vivax indicating a conserved role for the extended PRESAN domain of these proteins in targeting to the erythrocyte periphery.
date: 2014-08-11
official_url: http://dx.doi.org/10.1016/j.molbiopara.2014.07.011
vfaculties: VFLS
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: JOURNAL ARTICLE
verified: verified_manual
elements_source: PubMed
elements_id: 971663
doi: 10.1016/j.molbiopara.2014.07.011
pii: S0166-6851(14)00089-9
language_elements: ENG
lyricists_name: Osborne, Andrew
lyricists_id: AROSB74
full_text_status: public
publication: Mol Biochem Parasitol
volume: 196
number: 1
pagerange: 29-40
citation:        Tarr, SJ;    Moon, RW;    Hardege, I;    Osborne, AR;      (2014)    A conserved domain targets exported PHISTb family proteins to the periphery of Plasmodium infected erythrocytes.                   Mol Biochem Parasitol , 196  (1)   pp. 29-40.    10.1016/j.molbiopara.2014.07.011 <https://doi.org/10.1016/j.molbiopara.2014.07.011>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1443051/1/1-s2.0-S0166685114000899-main.pdf