eprintid: 1433921
rev_number: 38
eprint_status: archive
userid: 608
dir: disk0/01/43/39/21
datestamp: 2014-07-04 19:58:38
lastmod: 2024-06-17 16:12:38
status_changed: 2016-08-12 11:27:36
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Yusuf, Mohammed
creators_name: Parmar, Neha
creators_name: Bhella, Gurdeep K
creators_name: Robinson, Ian K
title: A Simple Filtration Technique for Obtaining Purified Human Chromosomes in Suspension
ispublished: pub
divisions: UCL
divisions: B04
divisions: C06
divisions: F64
keywords: chromosome, cytoplasmic debris, filtration, mitotic, nuclei,
note: © The Author(s), 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/
abstract: Here we present a simple method for cleaning polyamine human mitotic chromosomes in solution. This was achieved by filtering intact (unburst) nuclei along with both large and small cytoplasmic debris through a series of different pore sized filters. Pure human chromosomes were recovered using a simple reverse filtration step. Fluorescence microscopy was used to validate the chromosome suspension after each filtration step. This reverse filtration technique is an improvement in both procedure time and chromosome recovery compared to currently used post-purification methods. Chromosomes purified by our method could be used for many applications, such as structural studies using microfluidics and high resolution imaging or generation of chromosome paints and sequencing after flow cytometry.
date: 2014-04-03
official_url: https://doi.org/10.2144/000114168
vfaculties: VMPS
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
verified: verified_manual
elements_source: PubMed
elements_id: 960203
doi: 10.2144/000114168
pii: 000114168
lyricists_name: Parmar, Neha
lyricists_name: Robinson, Ian
lyricists_id: NPARM59
lyricists_id: IKROB22
full_text_status: public
publication: BioTechniques
volume: 56
number: 5
pagerange: 257-261
event_location: England
issn: 1940-9818
citation:        Yusuf, Mohammed;    Parmar, Neha;    Bhella, Gurdeep K;    Robinson, Ian K;      (2014)    A Simple Filtration Technique for Obtaining Purified Human Chromosomes in Suspension.                   BioTechniques , 56  (5)   pp. 257-261.    10.2144/000114168 <https://doi.org/10.2144/000114168>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1433921/1/Robinson_Yusuf_BTN_2014.pdf