eprintid: 1433921 rev_number: 38 eprint_status: archive userid: 608 dir: disk0/01/43/39/21 datestamp: 2014-07-04 19:58:38 lastmod: 2024-06-17 16:12:38 status_changed: 2016-08-12 11:27:36 type: article metadata_visibility: show item_issues_count: 0 creators_name: Yusuf, Mohammed creators_name: Parmar, Neha creators_name: Bhella, Gurdeep K creators_name: Robinson, Ian K title: A Simple Filtration Technique for Obtaining Purified Human Chromosomes in Suspension ispublished: pub divisions: UCL divisions: B04 divisions: C06 divisions: F64 keywords: chromosome, cytoplasmic debris, filtration, mitotic, nuclei, note: © The Author(s), 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/ abstract: Here we present a simple method for cleaning polyamine human mitotic chromosomes in solution. This was achieved by filtering intact (unburst) nuclei along with both large and small cytoplasmic debris through a series of different pore sized filters. Pure human chromosomes were recovered using a simple reverse filtration step. Fluorescence microscopy was used to validate the chromosome suspension after each filtration step. This reverse filtration technique is an improvement in both procedure time and chromosome recovery compared to currently used post-purification methods. Chromosomes purified by our method could be used for many applications, such as structural studies using microfluidics and high resolution imaging or generation of chromosome paints and sequencing after flow cytometry. date: 2014-04-03 official_url: https://doi.org/10.2144/000114168 vfaculties: VMPS oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_source: PubMed elements_id: 960203 doi: 10.2144/000114168 pii: 000114168 lyricists_name: Parmar, Neha lyricists_name: Robinson, Ian lyricists_id: NPARM59 lyricists_id: IKROB22 full_text_status: public publication: BioTechniques volume: 56 number: 5 pagerange: 257-261 event_location: England issn: 1940-9818 citation: Yusuf, Mohammed; Parmar, Neha; Bhella, Gurdeep K; Robinson, Ian K; (2014) A Simple Filtration Technique for Obtaining Purified Human Chromosomes in Suspension. BioTechniques , 56 (5) pp. 257-261. 10.2144/000114168 <https://doi.org/10.2144/000114168>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1433921/1/Robinson_Yusuf_BTN_2014.pdf