TY  - JOUR
JF  - BioTechniques
ID  - discovery1433921
UR  - https://doi.org/10.2144/000114168
N1  - © The Author(s), 2014. This is an Open Access article distributed under the terms of the Creative Commons Attribution Licence (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. https://creativecommons.org/licenses/by/4.0/
AV  - public
SN  - 1940-9818
N2  - Here we present a simple method for cleaning polyamine human mitotic chromosomes in solution. This was achieved by filtering intact (unburst) nuclei along with both large and small cytoplasmic debris through a series of different pore sized filters. Pure human chromosomes were recovered using a simple reverse filtration step. Fluorescence microscopy was used to validate the chromosome suspension after each filtration step. This reverse filtration technique is an improvement in both procedure time and chromosome recovery compared to currently used post-purification methods. Chromosomes purified by our method could be used for many applications, such as structural studies using microfluidics and high resolution imaging or generation of chromosome paints and sequencing after flow cytometry.
SP  - 257
VL  - 56
Y1  - 2014/04/03/
TI  - A Simple Filtration Technique for Obtaining Purified Human Chromosomes in Suspension
IS  - 5
A1  - Yusuf, Mohammed
A1  - Parmar, Neha
A1  - Bhella, Gurdeep K
A1  - Robinson, Ian K
KW  - chromosome
KW  -  cytoplasmic debris
KW  -  filtration
KW  -  mitotic
KW  -  nuclei
EP  - 261
ER  -