eprintid: 1432789 rev_number: 25 eprint_status: archive userid: 608 dir: disk0/01/43/27/89 datestamp: 2014-06-19 19:14:37 lastmod: 2021-09-17 22:48:37 status_changed: 2014-06-19 19:14:37 type: article metadata_visibility: show item_issues_count: 0 creators_name: Jin, GZ creators_name: Park, JH creators_name: Lee, EJ creators_name: Wall, IB creators_name: Kim, HW title: Utilizing PCL Microcarriers for High-Purity Isolation of Primary Endothelial Cells for Tissue Engineering. ispublished: pub divisions: UCL divisions: B04 divisions: C05 note: Copyright© Mary Ann Liebert, Inc. This work is licensed under a Creative Commons Attribution 3.0 United States License. You are free to copy, distribute, transmit and adapt this work, but you must attribute this work as ‘‘Tissue Engineering, Part C. Copyright 2014 Mary Ann Liebert, Inc. http://liebertpub.com/tec, used under a Creative Commons Attribution License: http://creativecommons.org/licenses/by/3.0/us/’’ abstract: Endothelial cells (ECs) are widely used in research, both for fundamental vascular biology research and for exploring strategies to create engineered vascularized tissues. Primary isolation often results in contamination from fibroblasts and vascular smooth muscle cells that can potentially affect function, particularly during the initial expansion period needed to establish the cell culture. In the current study, we explored the use of microcarriers to selectively isolate ECs from the lumen of intact vessels to enhance the purity during the isolation procedure. First, rat aortic explant culture was performed and after 2 weeks of culture, flow cytometry revealed that only 60% of the expanded cell population was positive for the endothelial marker CD31. Then, we employed a strategy to selectively isolate ECs and improve their purity by introducing microcarriers to the lumen of intact aorta. After 10 days, microcarriers were carefully removed and placed in cell culture dishes and at 15 days, a large near confluent layer of primary ECs populated the dish. Flow cytometry revealed that >90% of the expanded cells expressed CD31. Moreover, the cells were capable of forming tubule-like structures when plated onto Matrigel, confirming their function also. The highly modular and transportable nature of microcarriers has significant potential for isolating ECs at high purity, with minimal contamination. date: 2014-06-16 official_url: http://dx.doi.org/10.1089/ten.TEC.2013.0348 vfaculties: VENG oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green article_type_text: JOURNAL ARTICLE verified: verified_manual elements_source: PubMed elements_id: 952601 doi: 10.1089/ten.TEC.2013.0348 language_elements: ENG lyricists_name: Wall, Ivan lyricists_id: IWALL43 full_text_status: public publication: Tissue Eng Part C Methods volume: 20 number: 9 pagerange: 761-768 citation: Jin, GZ; Park, JH; Lee, EJ; Wall, IB; Kim, HW; (2014) Utilizing PCL Microcarriers for High-Purity Isolation of Primary Endothelial Cells for Tissue Engineering. Tissue Eng Part C Methods , 20 (9) pp. 761-768. 10.1089/ten.TEC.2013.0348 <https://doi.org/10.1089/ten.TEC.2013.0348>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1432789/1/ten.tec.2013.0348.pdf