%T A semi-automated technique for labeling and counting of apoptosing retinal cells %V 15 %A M Bizrah %A SC Dakin %A L Guo %A F Rahman %A M Parnell %A E Normando %A S Nizari %A B Davis %A A Younis %A MF Cordeiro %N 1 %P 169 -180 %D 2014 %J BMC Bioinformatics %L discovery1432362 %X Retinal ganglion cell (RGC) loss is one of the earliest and most important cellular changes in glaucoma. The DARC (Detection of Apoptosing Retinal Cells) technology enables in vivo real-time non-invasive imaging of single apoptosing retinal cells in animal models of glaucoma and Alzheimer's disease. To date, apoptosing RGCs imaged using DARC have been counted manually. This is time-consuming, labour-intensive, vulnerable to bias, and has considerable inter- and intra-operator variability %O © 2014 Bizrah et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.