TY - JOUR N2 - Retinal ganglion cell (RGC) loss is one of the earliest and most important cellular changes in glaucoma. The DARC (Detection of Apoptosing Retinal Cells) technology enables in vivo real-time non-invasive imaging of single apoptosing retinal cells in animal models of glaucoma and Alzheimer's disease. To date, apoptosing RGCs imaged using DARC have been counted manually. This is time-consuming, labour-intensive, vulnerable to bias, and has considerable inter- and intra-operator variability IS - 1 UR - http://dx.doi.org/10.1186/1471-2105-15-169 ID - discovery1432362 EP - 180 TI - A semi-automated technique for labeling and counting of apoptosing retinal cells Y1 - 2014/06/05/ AV - public JF - BMC Bioinformatics A1 - Bizrah, M A1 - Dakin, SC A1 - Guo, L A1 - Rahman, F A1 - Parnell, M A1 - Normando, E A1 - Nizari, S A1 - Davis, B A1 - Younis, A A1 - Cordeiro, MF N1 - © 2014 Bizrah et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. VL - 15 SP - 169 ER -