@article{discovery1432362, month = {June}, volume = {15}, pages = {169 --180}, note = {{\copyright} 2014 Bizrah et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.}, number = {1}, journal = {BMC Bioinformatics}, year = {2014}, title = {A semi-automated technique for labeling and counting of apoptosing retinal cells}, url = {http://dx.doi.org/10.1186/1471-2105-15-169}, abstract = {Retinal ganglion cell (RGC) loss is one of the earliest and most important cellular changes in glaucoma. The DARC (Detection of Apoptosing Retinal Cells) technology enables in vivo real-time non-invasive imaging of single apoptosing retinal cells in animal models of glaucoma and Alzheimer's disease. To date, apoptosing RGCs imaged using DARC have been counted manually. This is time-consuming, labour-intensive, vulnerable to bias, and has considerable inter- and intra-operator variability}, author = {Bizrah, M and Dakin, SC and Guo, L and Rahman, F and Parnell, M and Normando, E and Nizari, S and Davis, B and Younis, A and Cordeiro, MF} }