eprintid: 1429841
rev_number: 43
eprint_status: archive
userid: 608
dir: disk0/01/42/98/41
datestamp: 2014-05-14 18:39:00
lastmod: 2021-10-18 00:05:49
status_changed: 2016-04-05 12:28:21
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Yun, MH
creators_name: Gates, PB
creators_name: Brockes, JP
title: Sustained ERK activation underlies reprogramming in regeneration-competent salamander cells and distinguishes them from their mammalian counterparts
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D09
divisions: G03
keywords: Animals, Cell Cycle, Cell Differentiation, Cell Line, Extracellular Signal-Regulated MAP Kinases, Mammals, Mitogen-Activated Protein Kinases, Muscle Fibers, Skeletal, Regeneration, Urodela
note: © 2014 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
abstract: In regeneration-competent vertebrates, such as salamanders, regeneration depends on the ability of various differentiated adult cell types to undergo natural reprogramming. This ability is rarely observed in regeneration-incompetent species such as mammals, providing an explanation for their poor regenerative potential. To date, little is known about the molecular mechanisms mediating natural reprogramming during regeneration. Here, we have identified the extent of extracellular signal-regulated kinase (ERK) activation as a key component of such mechanisms. We show that sustained ERK activation following serum induction is required for re-entry into the cell cycle of postmitotic salamander muscle cells, partially by promoting the downregulation of p53 activity. Moreover, ERK activation induces epigenetic modifications and downregulation of muscle-specific genes such as Sox6. Remarkably, while long-term ERK activation is found in salamander myotubes, only transient activation is seen in their mammalian counterparts, suggesting that the extent of ERK activation could underlie differences in regenerative competence between species.
date: 2014-07-08
official_url: http://dx.doi.org/10.1016/j.stemcr.2014.05.009
vfaculties: VFLS
oa_status: green
full_text_type: pub
primo: open
primo_central: open_green
article_type_text: Journal Article
verified: verified_manual
elements_source: PubMed
elements_id: 946905
doi: 10.1016/j.stemcr.2014.05.009
pii: S2213-6711(14)00150-7
lyricists_name: Brockes, Jeremy
lyricists_name: Gates, Phillip
lyricists_name: Yun, Maximina
lyricists_id: JPBRO93
lyricists_id: PGATE52
lyricists_id: MYUNX04
full_text_status: public
publication: Stem Cell Reports
volume: 3
number: 1
pagerange: 15-23
event_location: United States
issn: 2213-6711
citation:        Yun, MH;    Gates, PB;    Brockes, JP;      (2014)    Sustained ERK activation underlies reprogramming in regeneration-competent salamander cells and distinguishes them from their mammalian counterparts.                   Stem Cell Reports , 3  (1)   pp. 15-23.    10.1016/j.stemcr.2014.05.009 <https://doi.org/10.1016/j.stemcr.2014.05.009>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1429841/1/PIIS2213671114001507.pdf