eprintid: 1426316 rev_number: 29 eprint_status: archive userid: 608 dir: disk0/01/42/63/16 datestamp: 2014-04-07 19:01:12 lastmod: 2021-10-18 00:16:52 status_changed: 2014-04-07 19:01:12 type: article metadata_visibility: show item_issues_count: 0 creators_name: Harris, AR creators_name: Daeden, A creators_name: Charras, GT title: Formation of adherens junctions leads to the emergence of a tissue-level tension in epithelial monolayers. ispublished: pub divisions: UCL divisions: B04 divisions: C06 divisions: F64 note: © 2014. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. abstract: Adherens junctions and desmosomes interface the cytoskeletons of adjacent cells into a mechanical syncitium. In doing so, intercellular junctions endow tissues with the strength needed to sustain mechanical stresses encountered in normal physiology and coordinate tension during morphogenesis. Though much is known about the biological mechanisms underlying junction formation, little is known about how tissue-scale mechanical properties are established. Here, we use deep AFM indentation to measure the apparent stiffness of epithelial monolayers reforming from dissociated cells and examine which cellular processes give rise to tissue-scale mechanics. We show that the formation of intercellular junctions coincided with an increase in the apparent stiffness of reforming monolayers that reflected the generation of a tissue-level tension. Tension rapidly increased reaching a maximum after 150 minutes before settling to a lower level over the next three hours as monolayers reached homeostasis. The emergence of tissue tension correlated with the formation of adherens junctions but not desmosomes. As a consequence, inhibition of any of the molecular mechanisms participating in adherens junction initiation, remodelling, and maturation significantly impeded the emergence of tissue-level tension in monolayers. date: 2014-03-21 official_url: http://dx.doi.org/10.1242/jcs.142349 vfaculties: VMPS oa_status: green full_text_type: pub primo: open primo_central: open_green article_type_text: JOURNAL ARTICLE verified: verified_manual elements_source: PubMed elements_id: 939339 doi: 10.1242/jcs.142349 pii: jcs.142349 language_elements: ENG lyricists_name: Charras, Guillaume lyricists_id: GCHAR39 full_text_status: public publication: J Cell Sci volume: 127 pagerange: 2507-2517 citation: Harris, AR; Daeden, A; Charras, GT; (2014) Formation of adherens junctions leads to the emergence of a tissue-level tension in epithelial monolayers. J Cell Sci , 127 pp. 2507-2517. 10.1242/jcs.142349 <https://doi.org/10.1242/jcs.142349>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1426316/1/J_Cell_Sci-2014-Harris-2507-17.pdf