eprintid: 1423663
rev_number: 40
eprint_status: archive
userid: 608
dir: disk0/01/42/36/63
datestamp: 2014-03-22 06:55:50
lastmod: 2021-10-10 22:58:02
status_changed: 2016-08-26 13:36:27
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Ibrahim, A
creators_name: Li, D
creators_name: Collins, A
creators_name: Tabakow, P
creators_name: Raisman, G
creators_name: Li, Y
title: Comparison of olfactory bulbar and mucosal cultures in a rat rhizotomy model
ispublished: pub
divisions: UCL
divisions: B02
divisions: C07
divisions: D07
divisions: F82
keywords: Animals, Cell Transplantation, Cells, Cultured, Disease Models, Animal, Female, Olfactory Bulb, Olfactory Mucosa, Rats, Rhizotomy, Spinal Cord Injuries
note: This is an Open Access article published under a Creative Commons Attribution Non-Commercial (CC BY NC) license (http://creativecommons.org/licenses/by-nc/3.0/).
abstract: In an ongoing clinical trial, a spinal injured patient who received a transplant of autologous cells cultured from the olfactory bulb is showing greater functional benefit than three previous patients with transplants of mucosal origin. Previous laboratory studies of transplantation into rat spinal cord injuries show that the superior reparative benefits of bulbar over mucosal cultures are associated with regeneration of severed corticospinal tract fibers over a bridge of olfactory ensheathing cells (OECs) formed across the injury site. In a rat rhizotomy paradigm, we reported that transplantation of bulbar cell cultures also enables severed axons of the C6-T1 dorsal roots to regenerate across a bridge of OECs into the spinal cord and restore electrophysiological transmission and forepaw grasping during a climbing test. We now report a repeat of the same rhizotomy procedure in 25 rats receiving cells cultured from olfactory mucosal biopsies. In no case did the transplanted cells form a bridging pathway. No axons crossed from the severed roots to the spinal cord, and there was no restoration of forepaw grasping. This suggests that the superior clinical benefit in the patient receiving bulbar cell transplants is due to regeneration of severed fibers across the injury site, and this correlates with imaging and the pattern of functional recovery. Using present culture protocols, the yield of OECs from bulbar biopsies is around 50%, but that from mucosal biopsies is less than 5%. Improving the yield of OECs from mucosal biopsies might avoid the necessity for the intracranial approach to obtain bulbar cells.
date: 2014
official_url: http://dx.doi.org/10.3727/096368913X676213
vfaculties: VFBRS
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: Comparative Study
verified: verified_manual
elements_source: PubMed
elements_id: 922506
doi: 10.3727/096368913X676213
pii: content-ct1088Ibrahim
lyricists_name: Collins, Andrew
lyricists_name: Li, Daqing
lyricists_name: Li, Ying
lyricists_name: Raisman, Geoffrey
lyricists_id: ACOLL47
lyricists_id: DLIXX89
lyricists_id: YLIXX26
lyricists_id: GRAIS26
full_text_status: public
publication: Cell Transplantation
volume: 23
number: 11
pagerange: 1465-1470
event_location: United States
issn: 1555-3892
citation:        Ibrahim, A;    Li, D;    Collins, A;    Tabakow, P;    Raisman, G;    Li, Y;      (2014)    Comparison of olfactory bulbar and mucosal cultures in a rat rhizotomy model.                   Cell Transplantation , 23  (11)   pp. 1465-1470.    10.3727/096368913X676213 <https://doi.org/10.3727/096368913X676213>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1423663/1/Li_s9.pdf