%0 Journal Article
%@ 1362-4962
%A Pancevac, C
%A Goldstone, DC
%A Ramos, A
%A Taylor, IA
%D 2010
%F discovery:1418595
%J Nucleic Acids Research
%K transcription, genetic, rna, complex
%N 9
%P 3119-3132
%T Structure of the Rna15 RRM-RNA complex reveals the molecular basis of GU specificity in transcriptional 3'-end processing factors
%U https://discovery.ucl.ac.uk/id/eprint/1418595/
%V 38
%X Rna15 is a core subunit of cleavage factor IA (CFIA),  an essential transcriptional 30  -end processing factor  from Saccharomyces cerevisiae. CFIA is required  for polyA site selection/cleavage targeting RNA  sequences that surround polyadenylation sites in  the 30  -UTR of RNA polymerase-II transcripts. RNA  recognition by CFIA is mediated by an RNA recognition  motif (RRM) contained in the Rna15 subunit of  the complex. We show here that Rna15 has a strong  and unexpected preference for GU containing RNAs  and reveal the molecular basis for a base selectivity  mechanism that accommodates G or U but discriminates  against C and A bases. This mode of base  selectivity is rather different to that observed in  other RRM-RNA structures and is structurally  conserved in CstF64, the mammalian counterpart  of Rna15. Our observations provide evidence for a  highly conserved mechanism of base recognition  amongst the 30  -end processing complexes that  interact with the U-rich or U/G-rich elements at  30  -end cleavage/polyadenylation sites.
%Z The Author(s) 2010. Published by Oxford University Press.  This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/  by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.