@article{discovery1418595,
            note = {The Author(s) 2010. Published by Oxford University Press.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/
by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.},
          volume = {38},
           pages = {3119--3132},
           month = {May},
          number = {9},
         journal = {Nucleic Acids Research},
           title = {Structure of the Rna15 RRM-RNA complex reveals the molecular basis of GU specificity in transcriptional 3'-end processing factors},
            year = {2010},
        abstract = {Rna15 is a core subunit of cleavage factor IA (CFIA),
an essential transcriptional 30
-end processing factor
from Saccharomyces cerevisiae. CFIA is required
for polyA site selection/cleavage targeting RNA
sequences that surround polyadenylation sites in
the 30
-UTR of RNA polymerase-II transcripts. RNA
recognition by CFIA is mediated by an RNA recognition
motif (RRM) contained in the Rna15 subunit of
the complex. We show here that Rna15 has a strong
and unexpected preference for GU containing RNAs
and reveal the molecular basis for a base selectivity
mechanism that accommodates G or U but discriminates
against C and A bases. This mode of base
selectivity is rather different to that observed in
other RRM-RNA structures and is structurally
conserved in CstF64, the mammalian counterpart
of Rna15. Our observations provide evidence for a
highly conserved mechanism of base recognition
amongst the 30
-end processing complexes that
interact with the U-rich or U/G-rich elements at
30
-end cleavage/polyadenylation sites.},
            issn = {1362-4962},
          author = {Pancevac, C and Goldstone, DC and Ramos, A and Taylor, IA},
             url = {http://doi.org/10.1093/nar/gkq002},
        keywords = {transcription, genetic, rna, complex}
}