eprintid: 1417579
rev_number: 34
eprint_status: archive
userid: 608
dir: disk0/01/41/75/79
datestamp: 2014-01-15 10:24:33
lastmod: 2021-11-07 23:51:10
status_changed: 2014-01-15 10:24:33
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Said, J
creators_name: Dodoo, CC
creators_name: Walker, M
creators_name: Parsons, D
creators_name: Stapleton, P
creators_name: Beezer, AE
creators_name: Gaisford, S
title: An in vitro test of the efficacy of silver-containing wound dressings against Staphylococcus aureus and Pseudomonas aeruginosa in simulated wound fluid
ispublished: pub
divisions: UCL
divisions: B02
divisions: C08
divisions: D10
divisions: G09
divisions: G08
keywords: Isothermal calorimetry, Pseudomonas aeruginosa, Silver, Efficacy, Staphylococcus aureus, Wound dressings
note: This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License (CC BY-NC-ND), which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
abstract: An isothermal microcalorimetric assay was used to quantify the efficacy of a silver-containing wound dressing against two common wound pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. The growth patterns of the two species were unique and varied depending on the environment in which the organisms were grown. Addition of non-silver-containing dressing altered the growth kinetics while addition of silver (contained either in a dressing or as AgNO3 solution) was seen to elicit inhibition and/or kill depending on concentration. Tests were conducted in nutrient broth and simulated wound fluid. It was found that minimum inhibitory and minimum bactericidal concentration values were higher in simulated wound fluid and under anaerobic conditions. Bioavailability of silver from the wound dressing was 35% against S. aureus in nutrient broth and 68% against both species in simulated wound fluid. The data highlight the importance of developing and conducting in vitro assays in biorelevant media.
date: 2014-02-28
official_url: http://dx.doi.org/10.1016/j.ijpharm.2013.12.037
vfaculties: VFLS
oa_status: green
full_text_type: pub
language: eng
primo: open
primo_central: open_green
article_type_text: JOURNAL ARTICLE
verified: verified_manual
elements_source: PubMed
elements_id: 922427
doi: 10.1016/j.ijpharm.2013.12.037
pii: S0378-5173(13)01113-7
language_elements: ENG
lyricists_name: Gaisford, Simon
lyricists_name: Stapleton, Paul
lyricists_id: SGAIS88
lyricists_id: PDSTA67
full_text_status: public
publication: International Journal of Pharmaceutics
volume: 462
number: 1-2
pagerange: 123-128
issn: 0378-5173
citation:        Said, J;    Dodoo, CC;    Walker, M;    Parsons, D;    Stapleton, P;    Beezer, AE;    Gaisford, S;      (2014)    An in vitro test of the efficacy of silver-containing wound dressings against Staphylococcus aureus and Pseudomonas aeruginosa in simulated wound fluid.                   International Journal of Pharmaceutics , 462  (1-2)   pp. 123-128.    10.1016/j.ijpharm.2013.12.037 <https://doi.org/10.1016/j.ijpharm.2013.12.037>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1417579/2/IJP%20462%20%282014%29%20123-128.pdf