eprintid: 1417579 rev_number: 34 eprint_status: archive userid: 608 dir: disk0/01/41/75/79 datestamp: 2014-01-15 10:24:33 lastmod: 2021-11-07 23:51:10 status_changed: 2014-01-15 10:24:33 type: article metadata_visibility: show item_issues_count: 0 creators_name: Said, J creators_name: Dodoo, CC creators_name: Walker, M creators_name: Parsons, D creators_name: Stapleton, P creators_name: Beezer, AE creators_name: Gaisford, S title: An in vitro test of the efficacy of silver-containing wound dressings against Staphylococcus aureus and Pseudomonas aeruginosa in simulated wound fluid ispublished: pub divisions: UCL divisions: B02 divisions: C08 divisions: D10 divisions: G09 divisions: G08 keywords: Isothermal calorimetry, Pseudomonas aeruginosa, Silver, Efficacy, Staphylococcus aureus, Wound dressings note: This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-No Derivative Works License (CC BY-NC-ND), which permits non-commercial use, distribution, and reproduction in any medium, provided the original author and source are credited. abstract: An isothermal microcalorimetric assay was used to quantify the efficacy of a silver-containing wound dressing against two common wound pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. The growth patterns of the two species were unique and varied depending on the environment in which the organisms were grown. Addition of non-silver-containing dressing altered the growth kinetics while addition of silver (contained either in a dressing or as AgNO3 solution) was seen to elicit inhibition and/or kill depending on concentration. Tests were conducted in nutrient broth and simulated wound fluid. It was found that minimum inhibitory and minimum bactericidal concentration values were higher in simulated wound fluid and under anaerobic conditions. Bioavailability of silver from the wound dressing was 35% against S. aureus in nutrient broth and 68% against both species in simulated wound fluid. The data highlight the importance of developing and conducting in vitro assays in biorelevant media. date: 2014-02-28 official_url: http://dx.doi.org/10.1016/j.ijpharm.2013.12.037 vfaculties: VFLS oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green article_type_text: JOURNAL ARTICLE verified: verified_manual elements_source: PubMed elements_id: 922427 doi: 10.1016/j.ijpharm.2013.12.037 pii: S0378-5173(13)01113-7 language_elements: ENG lyricists_name: Gaisford, Simon lyricists_name: Stapleton, Paul lyricists_id: SGAIS88 lyricists_id: PDSTA67 full_text_status: public publication: International Journal of Pharmaceutics volume: 462 number: 1-2 pagerange: 123-128 issn: 0378-5173 citation: Said, J; Dodoo, CC; Walker, M; Parsons, D; Stapleton, P; Beezer, AE; Gaisford, S; (2014) An in vitro test of the efficacy of silver-containing wound dressings against Staphylococcus aureus and Pseudomonas aeruginosa in simulated wound fluid. International Journal of Pharmaceutics , 462 (1-2) pp. 123-128. 10.1016/j.ijpharm.2013.12.037 <https://doi.org/10.1016/j.ijpharm.2013.12.037>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1417579/2/IJP%20462%20%282014%29%20123-128.pdf