<> <http://www.w3.org/2000/01/rdf-schema#comment> "The repository administrator has not yet configured an RDF license."^^<http://www.w3.org/2001/XMLSchema#string> . <> <http://xmlns.com/foaf/0.1/primaryTopic> <https://discovery.ucl.ac.uk/id/eprint/1401337> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/AcademicArticle> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Article> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/dc/terms/title> "Cysteine Methylation Controls Radical Generation in the Cfr Radical AdoMet rRNA Methyltransferase"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/ontology/bibo/abstract> "The 'radical S-adenosyl-L-methionine (AdoMet)' enzyme Cfr methylates adenosine 2503 of the 23S rRNA in the peptidyltransferase centre (P-site) of the bacterial ribosome. This modification protects host bacteria, notably methicillin-resistant Staphylococcus aureus (MRSA), from numerous antibiotics, including agents (e.g. linezolid, retapamulin) that were developed to treat such organisms. Cfr contains a single [4Fe-4S] cluster that binds two separate molecules of AdoMet during the reaction cycle. These are used sequentially to first methylate a cysteine residue, Cys338; and subsequently generate an oxidative radical intermediate that facilitates methyl transfer to the unreactive C8 (and/or C2) carbon centres of adenosine 2503. How the Cfr active site, with its single [4Fe-4S] cluster, catalyses these two distinct activities that each utilise AdoMet as a substrate remains to be established. Here, we use absorbance and electron paramagnetic resonance (EPR) spectroscopy to investigate the interactions of AdoMet with the [4Fe-4S] clusters of wild-type Cfr and a Cys338 Ala mutant, which is unable to accept a methyl group. Cfr binds AdoMet with high (∼ 10 µM) affinity notwithstanding the absence of the RNA cosubstrate. In wild-type Cfr, where Cys338 is methylated, AdoMet binding leads to rapid oxidation of the [4Fe-4S] cluster and production of 5'-deoxyadenosine (DOA). In contrast, while Cys338 Ala Cfr binds AdoMet with equivalent affinity, oxidation of the [4Fe-4S] cluster is not observed. Our results indicate that the presence of a methyl group on Cfr Cys338 is a key determinant of the activity of the enzyme towards AdoMet, thus enabling a single active site to support two distinct modes of AdoMet cleavage."^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/dc/terms/date> "2013-07-05" . <https://discovery.ucl.ac.uk/id/document/59024> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Document> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/ontology/bibo/volume> "8" . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/ontology/bibo/issue> "7" . <https://discovery.ucl.ac.uk/id/publication/ext-19326203> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://purl.org/ontology/bibo/Collection> . <https://discovery.ucl.ac.uk/id/publication/ext-19326203> <http://xmlns.com/foaf/0.1/name> "PLoS One"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/publication/ext-19326203> . <https://discovery.ucl.ac.uk/id/publication/ext-19326203> <http://www.w3.org/2002/07/owl#sameAs> <urn:issn:19326203> . <https://discovery.ucl.ac.uk/id/publication/ext-19326203> <http://purl.org/ontology/bibo/issn> "19326203" . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/ontology/bibo/status> <http://purl.org/ontology/bibo/status/published> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-2c378e4de221f4baa8fe4f7cbf5ea6be> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/1401337#authors> . <https://discovery.ucl.ac.uk/id/eprint/1401337#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_1> <https://discovery.ucl.ac.uk/id/person/ext-2c378e4de221f4baa8fe4f7cbf5ea6be> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-32fbaaf7924419435916a7e52c497651> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/1401337#authors> . <https://discovery.ucl.ac.uk/id/eprint/1401337#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_2> <https://discovery.ucl.ac.uk/id/person/ext-32fbaaf7924419435916a7e52c497651> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-fefccdb01fc30c05a1c352078db3b6d9> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/1401337#authors> . <https://discovery.ucl.ac.uk/id/eprint/1401337#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_3> <https://discovery.ucl.ac.uk/id/person/ext-fefccdb01fc30c05a1c352078db3b6d9> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-f96987da7f2e88d95495bc86ac59e90a> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/1401337#authors> . <https://discovery.ucl.ac.uk/id/eprint/1401337#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_4> <https://discovery.ucl.ac.uk/id/person/ext-f96987da7f2e88d95495bc86ac59e90a> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-617206dcfedf4c66cebb935089a0db2a> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/1401337#authors> . <https://discovery.ucl.ac.uk/id/eprint/1401337#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_5> <https://discovery.ucl.ac.uk/id/person/ext-617206dcfedf4c66cebb935089a0db2a> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/dc/terms/creator> <https://discovery.ucl.ac.uk/id/person/ext-32309952da34884b7e36a77c7225a80b> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/ontology/bibo/authorList> <https://discovery.ucl.ac.uk/id/eprint/1401337#authors> . <https://discovery.ucl.ac.uk/id/eprint/1401337#authors> <http://www.w3.org/1999/02/22-rdf-syntax-ns#_6> <https://discovery.ucl.ac.uk/id/person/ext-32309952da34884b7e36a77c7225a80b> . <https://discovery.ucl.ac.uk/id/person/ext-fefccdb01fc30c05a1c352078db3b6d9> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-fefccdb01fc30c05a1c352078db3b6d9> <http://xmlns.com/foaf/0.1/givenName> "RC"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-fefccdb01fc30c05a1c352078db3b6d9> <http://xmlns.com/foaf/0.1/familyName> "Driesener"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-fefccdb01fc30c05a1c352078db3b6d9> <http://xmlns.com/foaf/0.1/name> "RC Driesener"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-2c378e4de221f4baa8fe4f7cbf5ea6be> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-2c378e4de221f4baa8fe4f7cbf5ea6be> <http://xmlns.com/foaf/0.1/givenName> "MR"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-2c378e4de221f4baa8fe4f7cbf5ea6be> <http://xmlns.com/foaf/0.1/familyName> "Challand"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-2c378e4de221f4baa8fe4f7cbf5ea6be> <http://xmlns.com/foaf/0.1/name> "MR Challand"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-32fbaaf7924419435916a7e52c497651> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-32fbaaf7924419435916a7e52c497651> <http://xmlns.com/foaf/0.1/givenName> "E"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-32fbaaf7924419435916a7e52c497651> <http://xmlns.com/foaf/0.1/familyName> "Salvadori"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-32fbaaf7924419435916a7e52c497651> <http://xmlns.com/foaf/0.1/name> "E Salvadori"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-f96987da7f2e88d95495bc86ac59e90a> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-f96987da7f2e88d95495bc86ac59e90a> <http://xmlns.com/foaf/0.1/givenName> "CW"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-f96987da7f2e88d95495bc86ac59e90a> <http://xmlns.com/foaf/0.1/familyName> "Kay"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-f96987da7f2e88d95495bc86ac59e90a> <http://xmlns.com/foaf/0.1/name> "CW Kay"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-617206dcfedf4c66cebb935089a0db2a> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-617206dcfedf4c66cebb935089a0db2a> <http://xmlns.com/foaf/0.1/givenName> "PL"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-617206dcfedf4c66cebb935089a0db2a> <http://xmlns.com/foaf/0.1/familyName> "Roach"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-617206dcfedf4c66cebb935089a0db2a> <http://xmlns.com/foaf/0.1/name> "PL Roach"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-32309952da34884b7e36a77c7225a80b> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://xmlns.com/foaf/0.1/Person> . <https://discovery.ucl.ac.uk/id/person/ext-32309952da34884b7e36a77c7225a80b> <http://xmlns.com/foaf/0.1/givenName> "J"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-32309952da34884b7e36a77c7225a80b> <http://xmlns.com/foaf/0.1/familyName> "Spencer"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/person/ext-32309952da34884b7e36a77c7225a80b> <http://xmlns.com/foaf/0.1/name> "J Spencer"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/EPrint> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/ArticleEPrint> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://purl.org/dc/terms/isPartOf> <https://discovery.ucl.ac.uk/id/repository> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/59024> . <https://discovery.ucl.ac.uk/id/document/59024> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> . <https://discovery.ucl.ac.uk/id/document/59024> <http://www.w3.org/2000/01/rdf-schema#label> "Cysteine Methylation Controls Radical Generation in the Cfr Radical AdoMet rRNA Methyltransferase (PDF)"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/document/59024> <http://eprints.org/relation/hasVolatileVersion> <https://discovery.ucl.ac.uk/id/document/92957> . <https://discovery.ucl.ac.uk/id/document/59024> <http://eprints.org/relation/haspreviewThumbnailVersion> <https://discovery.ucl.ac.uk/id/document/92957> . <https://discovery.ucl.ac.uk/id/document/59024> <http://eprints.org/relation/hasVersion> <https://discovery.ucl.ac.uk/id/document/92957> . <https://discovery.ucl.ac.uk/id/document/59024> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/1401337/1/journal.pone.0067979.pdf> . <https://discovery.ucl.ac.uk/id/document/59024> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/1401337/1/journal.pone.0067979.pdf> . <https://discovery.ucl.ac.uk/id/eprint/1401337/1/journal.pone.0067979.pdf> <http://www.w3.org/2000/01/rdf-schema#label> "journal.pone.0067979.pdf"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/92957> . <https://discovery.ucl.ac.uk/id/document/92957> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> . <https://discovery.ucl.ac.uk/id/document/92957> <http://www.w3.org/2000/01/rdf-schema#label> "Cysteine Methylation Controls Radical Generation in the Cfr Radical AdoMet rRNA Methyltransferase (Image (PNG))"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/document/92957> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/59024> . <https://discovery.ucl.ac.uk/id/document/92957> <http://eprints.org/relation/ispreviewThumbnailVersionOf> <https://discovery.ucl.ac.uk/id/document/59024> . <https://discovery.ucl.ac.uk/id/document/92957> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/59024> . <https://discovery.ucl.ac.uk/id/document/92957> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/1401337/2/preview.png> . <https://discovery.ucl.ac.uk/id/document/92957> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/1401337/2/preview.png> . <https://discovery.ucl.ac.uk/id/eprint/1401337/2/preview.png> <http://www.w3.org/2000/01/rdf-schema#label> "preview.png"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://eprints.org/ontology/hasDocument> <https://discovery.ucl.ac.uk/id/document/119249> . <https://discovery.ucl.ac.uk/id/document/119249> <http://www.w3.org/1999/02/22-rdf-syntax-ns#type> <http://eprints.org/ontology/Document> . <https://discovery.ucl.ac.uk/id/document/119249> <http://www.w3.org/2000/01/rdf-schema#label> "Cysteine Methylation Controls Radical Generation in the Cfr Radical AdoMet rRNA Methyltransferase (Other)"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/document/119249> <http://eprints.org/relation/isVersionOf> <https://discovery.ucl.ac.uk/id/document/59024> . <https://discovery.ucl.ac.uk/id/document/119249> <http://eprints.org/relation/isVolatileVersionOf> <https://discovery.ucl.ac.uk/id/document/59024> . <https://discovery.ucl.ac.uk/id/document/119249> <http://eprints.org/relation/isIndexCodesVersionOf> <https://discovery.ucl.ac.uk/id/document/59024> . <https://discovery.ucl.ac.uk/id/document/119249> <http://eprints.org/ontology/hasFile> <https://discovery.ucl.ac.uk/id/eprint/1401337/3/indexcodes.txt> . <https://discovery.ucl.ac.uk/id/document/119249> <http://purl.org/dc/terms/hasPart> <https://discovery.ucl.ac.uk/id/eprint/1401337/3/indexcodes.txt> . <https://discovery.ucl.ac.uk/id/eprint/1401337/3/indexcodes.txt> <http://www.w3.org/2000/01/rdf-schema#label> "indexcodes.txt"^^<http://www.w3.org/2001/XMLSchema#string> . <https://discovery.ucl.ac.uk/id/eprint/1401337> <http://www.w3.org/2000/01/rdf-schema#seeAlso> <https://discovery.ucl.ac.uk/id/eprint/1401337/> . <https://discovery.ucl.ac.uk/id/eprint/1401337/> <http://purl.org/dc/elements/1.1/title> "HTML Summary of #1401337 \n\nCysteine Methylation Controls Radical Generation in the Cfr Radical AdoMet rRNA Methyltransferase\n\n" . <https://discovery.ucl.ac.uk/id/eprint/1401337/> <http://purl.org/dc/elements/1.1/format> "text/html" . <https://discovery.ucl.ac.uk/id/eprint/1401337/> 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