eprintid: 1395631 rev_number: 34 eprint_status: archive userid: 608 dir: disk0/01/39/56/31 datestamp: 2013-06-06 18:56:57 lastmod: 2021-10-21 00:06:11 status_changed: 2013-06-06 18:56:57 type: article metadata_visibility: show item_issues_count: 0 creators_name: Jones, BM creators_name: Iglesias-Rodriguez, MD creators_name: Skipp, PJ creators_name: Edwards, RJ creators_name: Greaves, MJ creators_name: Young, JR creators_name: Elderfield, H creators_name: O'Connor, CD title: Responses of the Emiliania huxleyi Proteome to Ocean Acidification ispublished: pub divisions: UCL divisions: B04 divisions: C06 divisions: F57 note: © 2013 Jones et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PubMed ID: 23593500 abstract: Ocean acidification due to rising atmospheric CO2 is expected to affect the physiology of important calcifying marine organisms, but the nature and magnitude of change is yet to be established. In coccolithophores, different species and strains display varying calcification responses to ocean acidification, but the underlying biochemical properties remain unknown. We employed an approach combining tandem mass-spectrometry with isobaric tagging (iTRAQ) and multiple database searching to identify proteins that were differentially expressed in cells of the marine coccolithophore species Emiliania huxleyi (strain NZEH) between two CO2 conditions: 395 (~current day) and ~1340 p.p.m.v. CO2. Cells exposed to the higher CO2 condition contained more cellular particulate inorganic carbon (CaCO3) and particulate organic nitrogen and carbon than those maintained in present-day conditions. These results are linked with the observation that cells grew slower under elevated CO2, indicating cell cycle disruption. Under high CO2 conditions, coccospheres were larger and cells possessed bigger coccoliths that did not show any signs of malformation compared to those from cells grown under present-day CO2 levels. No differences in calcification rate, particulate organic carbon production or cellular organic carbon: nitrogen ratios were observed. Results were not related to nutrient limitation or acclimation status of cells. At least 46 homologous protein groups from a variety of functional processes were quantified in these experiments, of which four (histones H2A, H3, H4 and a chloroplastic 30S ribosomal protein S7) showed down-regulation in all replicates exposed to high CO2, perhaps reflecting the decrease in growth rate. We present evidence of cellular stress responses but proteins associated with many key metabolic processes remained unaltered. Our results therefore suggest that this E. huxleyi strain possesses some acclimation mechanisms to tolerate future CO2 scenarios, although the observed decline in growth rate may be an overriding factor affecting the success of this ecotype in future oceans. date: 2013-04-12 official_url: http://dx.doi.org/10.1371/journal.pone.0061868 vfaculties: VMPS oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green verified: verified_manual elements_source: WoS-Lite elements_id: 871401 doi: 10.1371/journal.pone.0061868 lyricists_name: Young, Jeremy lyricists_id: JRYOU59 full_text_status: public publication: PLOS ONE volume: 8 number: 4 article_number: e61868 issn: 1932-6203 citation: Jones, BM; Iglesias-Rodriguez, MD; Skipp, PJ; Edwards, RJ; Greaves, MJ; Young, JR; Elderfield, H; Jones, BM; Iglesias-Rodriguez, MD; Skipp, PJ; Edwards, RJ; Greaves, MJ; Young, JR; Elderfield, H; O'Connor, CD; - view fewer <#> (2013) Responses of the Emiliania huxleyi Proteome to Ocean Acidification. PLOS ONE , 8 (4) , Article e61868. 10.1371/journal.pone.0061868 <https://doi.org/10.1371/journal.pone.0061868>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1395631/1/journal.pone.0061868.pdf