eprintid: 1395037 rev_number: 60 eprint_status: archive userid: 608 dir: disk0/01/39/50/37 datestamp: 2013-06-01 18:39:21 lastmod: 2021-09-20 22:17:05 status_changed: 2014-02-24 15:01:42 type: article metadata_visibility: show item_issues_count: 0 creators_name: Kolyva, C creators_name: Ghosh, A creators_name: Tachtsidis, I creators_name: Highton, D creators_name: Cooper, CE creators_name: Smith, M creators_name: Elwell, CE title: Cytochrome c oxidase response to changes in cerebral oxygen delivery in the adult brain shows higher brain-specificity than haemoglobin ispublished: pub divisions: UCL divisions: B02 divisions: C07 divisions: B04 divisions: C05 divisions: F42 keywords: Cytochrome c oxidase; Hypoxia; Hyperoxia; Hypocapnia; Hypercapnia; Near-infrared spectroscopy; note: © 2013 The Authors. Published by Elsevier Inc. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. abstract: The redox state of cerebral mitochondrial cytochrome c oxidase monitored with near-infrared spectroscopy (Δ[oxCCO]) is a signal with strong potential as a non-invasive, bedside biomarker of cerebral metabolic status. We hypothesised that the higher mitochondrial density of brain compared to skin and skull would lead to evidence of brain-specificity of the Δ[oxCCO] signal when measured with a multi-distance near-infrared spectroscopy (NIRS) system. Measurements of Δ[oxCCO] as well as of concentration changes in oxygenated (Δ[HbO2]) and deoxygenated haemoglobin (Δ[HHb]) were taken at multiple source-detector distances during systemic hypoxia and hypocapnia (decrease in cerebral oxygen delivery), and hyperoxia and hypercapnia (increase in cerebral oxygen delivery) from 15 adult healthy volunteers. Increasing source-detector spacing is associated with increasing light penetration depth and thus higher sensitivity to cerebral changes. An increase in Δ[oxCCO] was observed during the challenges that increased cerebral oxygen delivery and the opposite was observed when cerebral oxygen delivery decreased. A consistent pattern of statistically significant increasing amplitude of the Δ[oxCCO] response with increasing light penetration depth was observed in all four challenges, a behaviour that was distinctly different from that of the haemoglobin chromophores, which did not show this statistically significant depth gradient. This depth-dependence of the Δ[oxCCO] signal corroborates the notion of higher concentrations of CCO being present in cerebral tissue compared to extracranial components and highlights the value of NIRS-derived Δ[oxCCO] as a brain-specific signal of cerebral metabolism, superior in this aspect to haemoglobin. date: 2014-01-15 official_url: http://dx.doi.org/10.1016/j.neuroimage.2013.05.070 vfaculties: VFBRS vfaculties: VENG oa_status: green full_text_type: pub language: eng primo: open primo_central: open_green article_type_text: JOURNAL ARTICLE verified: verified_manual elements_source: PubMed elements_id: 874790 doi: 10.1016/j.neuroimage.2013.05.070 pii: S1053-8119(13)00570-3 language_elements: ENG lyricists_name: Elwell, Clare lyricists_name: Ghosh, Arnab lyricists_name: Highton, David lyricists_name: Kolyva, Christina lyricists_name: Smith, Martin lyricists_name: Tachtsidis, Ilias lyricists_id: CEELW72 lyricists_id: AGHOS94 lyricists_id: DHIGH82 lyricists_id: CKOLY70 lyricists_id: MSMIT43 lyricists_id: ITACH19 full_text_status: public publication: Neuroimage volume: 85 number: 1 pagerange: 234-244 issn: 1053-8119 citation: Kolyva, C; Ghosh, A; Tachtsidis, I; Highton, D; Cooper, CE; Smith, M; Elwell, CE; (2014) Cytochrome c oxidase response to changes in cerebral oxygen delivery in the adult brain shows higher brain-specificity than haemoglobin. Neuroimage , 85 (1) pp. 234-244. 10.1016/j.neuroimage.2013.05.070 <https://doi.org/10.1016/j.neuroimage.2013.05.070>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1395037/1/1-s2.0-S1053811913005703-main.pdf