eprintid: 1390683
rev_number: 54
eprint_status: archive
userid: 608
dir: disk0/01/39/06/83
datestamp: 2013-04-17 18:37:51
lastmod: 2021-09-20 00:02:46
status_changed: 2013-04-17 18:37:51
type: article
metadata_visibility: show
item_issues_count: 0
creators_name: Begum, R
creators_name: Powner, MB
creators_name: Hudson, N
creators_name: Hogg, C
creators_name: Jeffery, G
title: Treatment with 670 nm light up regulates cytochrome C oxidase expression and reduces inflammation in an age-related macular degeneration model.
ispublished: pub
divisions: UCL
divisions: B02
divisions: C07
divisions: D08
note: © 2013 Begum et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PMCID: PMC3585189
abstract: Inflammation is an umbrella feature of ageing. It is present in the aged retina and many retinal diseases including age-related macular degeneration (AMD). In ageing and in AMD mitochondrial function declines. In normal ageing this can be manipulated by brief exposure to 670 nm light on the retina, which increases mitochondrial membrane potential and reduces inflammation. Here we ask if 670 nm exposure has the same ability in an aged mouse model of AMD, the complement factor H knockout (CFH(-/-)) where inflammation is a key feature. Further, we ask whether this occurs when 670 nm is delivered briefly in environmental lighting rather than directly focussed on the retina. Mice were exposed to 670 nm for 6 minutes twice a day for 14 days in the form of supplemented environmental light. Exposed animals had significant increase in cytochrome c oxidase (COX), which is a mitochondrial enzyme regulating oxidative phosphorylation.There was a significant reduction in complement component C3, an inflammatory marker in the outer retina. Vimetin and glial fibrillary acidic protein (GFAP) expression, which reflect retinal stress in Muller glia, were also significantly down regulated. There were also significant changes in outer retinal macrophage morphology. However, amyloid beta (Aβ) load, which also increases with age in the outer retina and is pro-inflammatory, did not change. Hence, 670 nm is effective in reducing inflammation probably via COX activation in mice with a genotype similar to that in 50% of AMD patients even when brief exposures are delivered via environmental lighting. Further, inflammation can be reduced independent of Aβ. The efficacy revealed here supports current early stage clinical trials of 670 nm in AMD patients.
date: 2013-02-28
official_url: http://dx.doi.org/10.1371/journal.pone.0057828
oa_status: green
pmcid: PMC3585189
language: eng
primo: open
primo_central: open_green
article_type_text: Journal Article, Research Support, Non-U.S. Gov't
verified: verified_manual
elements_source: PubMed
elements_id: 855764
doi: 10.1371/journal.pone.0057828
pii: PONE-D-12-31171
lyricists_name: Begum, Rana
lyricists_name: Jeffery, Glen
lyricists_name: Powner, Michael
lyricists_id: RBEGU53
lyricists_id: GJEFF26
lyricists_id: MBPOW76
full_text_status: public
publication: PLoS One
volume: 8
number: 2
article_number: e57828
pagerange: -
event_location: United States
issn: 1932-6203
citation:        Begum, R;    Powner, MB;    Hudson, N;    Hogg, C;    Jeffery, G;      (2013)    Treatment with 670 nm light up regulates cytochrome C oxidase expression and reduces inflammation in an age-related macular degeneration model.                   PLoS One , 8  (2)    , Article e57828.  10.1371/journal.pone.0057828 <https://doi.org/10.1371/journal.pone.0057828>.       Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1390683/1/journal.pone.0057828.pdf