eprintid: 1390683 rev_number: 54 eprint_status: archive userid: 608 dir: disk0/01/39/06/83 datestamp: 2013-04-17 18:37:51 lastmod: 2021-09-20 00:02:46 status_changed: 2013-04-17 18:37:51 type: article metadata_visibility: show item_issues_count: 0 creators_name: Begum, R creators_name: Powner, MB creators_name: Hudson, N creators_name: Hogg, C creators_name: Jeffery, G title: Treatment with 670 nm light up regulates cytochrome C oxidase expression and reduces inflammation in an age-related macular degeneration model. ispublished: pub divisions: UCL divisions: B02 divisions: C07 divisions: D08 note: © 2013 Begum et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. PMCID: PMC3585189 abstract: Inflammation is an umbrella feature of ageing. It is present in the aged retina and many retinal diseases including age-related macular degeneration (AMD). In ageing and in AMD mitochondrial function declines. In normal ageing this can be manipulated by brief exposure to 670 nm light on the retina, which increases mitochondrial membrane potential and reduces inflammation. Here we ask if 670 nm exposure has the same ability in an aged mouse model of AMD, the complement factor H knockout (CFH(-/-)) where inflammation is a key feature. Further, we ask whether this occurs when 670 nm is delivered briefly in environmental lighting rather than directly focussed on the retina. Mice were exposed to 670 nm for 6 minutes twice a day for 14 days in the form of supplemented environmental light. Exposed animals had significant increase in cytochrome c oxidase (COX), which is a mitochondrial enzyme regulating oxidative phosphorylation.There was a significant reduction in complement component C3, an inflammatory marker in the outer retina. Vimetin and glial fibrillary acidic protein (GFAP) expression, which reflect retinal stress in Muller glia, were also significantly down regulated. There were also significant changes in outer retinal macrophage morphology. However, amyloid beta (Aβ) load, which also increases with age in the outer retina and is pro-inflammatory, did not change. Hence, 670 nm is effective in reducing inflammation probably via COX activation in mice with a genotype similar to that in 50% of AMD patients even when brief exposures are delivered via environmental lighting. Further, inflammation can be reduced independent of Aβ. The efficacy revealed here supports current early stage clinical trials of 670 nm in AMD patients. date: 2013-02-28 official_url: http://dx.doi.org/10.1371/journal.pone.0057828 oa_status: green pmcid: PMC3585189 language: eng primo: open primo_central: open_green article_type_text: Journal Article, Research Support, Non-U.S. Gov't verified: verified_manual elements_source: PubMed elements_id: 855764 doi: 10.1371/journal.pone.0057828 pii: PONE-D-12-31171 lyricists_name: Begum, Rana lyricists_name: Jeffery, Glen lyricists_name: Powner, Michael lyricists_id: RBEGU53 lyricists_id: GJEFF26 lyricists_id: MBPOW76 full_text_status: public publication: PLoS One volume: 8 number: 2 article_number: e57828 pagerange: - event_location: United States issn: 1932-6203 citation: Begum, R; Powner, MB; Hudson, N; Hogg, C; Jeffery, G; (2013) Treatment with 670 nm light up regulates cytochrome C oxidase expression and reduces inflammation in an age-related macular degeneration model. PLoS One , 8 (2) , Article e57828. 10.1371/journal.pone.0057828 <https://doi.org/10.1371/journal.pone.0057828>. Green open access document_url: https://discovery.ucl.ac.uk/id/eprint/1390683/1/journal.pone.0057828.pdf