TY  - JOUR
A1  - Watson, JA
A1  - Bryan, K
A1  - Williams, R
A1  - Popov, S
A1  - Vujanic, G
A1  - Coulomb, A
A1  - Boccon-Gibod, L
A1  - Graf, N
A1  - Pritchard-Jones, K
A1  - O'Sullivan, M
SN  - 1932-6203
N2  - The current SIOP treatment protocol for Wilms' tumor involves pre-operative chemotherapy followed by nephrectomy. Not all patients benefit equally from such chemotherapy. The aim of this study was to generate a miRNA profile of chemo resistant blastemal cells in high risk Wilms' tumors which might serve as predictive markers of therapeutic response at the pre-treatment biopsy stage. We have shown here that unsupervised hierarchical clustering of genome-wide miRNA expression profiles can clearly separate intermediate risk tumors from high risk tumors. A total of 29 miRNAs were significantly differentially expressed between post-treatment intermediate risk and high risk groups, including miRNAs that have been previously linked to chemo resistance in other cancer types. Furthermore, 7 of these 29 miRNAs were already at the pre-treatment biopsy stage differentially expressed between cases ultimately deemed intermediate risk compared to high risk. These miRNA alterations include down-regulation in high risk cases of miR-193a.5p, miR-27a and the up-regulation of miR-483.5p, miR-628.5p, miR-590.5p, miR-302a and miR-367. The demonstration of such miRNA markers at the pre-treatment biopsy stage could permit stratification of patients to more tailored treatment regimens.
IS  - 1
AV  - public
ID  - discovery1383814
Y1  - 2013///
UR  - http://dx.doi.org/10.1371/journal.pone.0053417
TI  - miRNA profiles as a predictor of chemoresponsiveness in Wilms' tumor blastema.
N1  - © 2013 Watson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

This project was funded by the Health Research Board (HRB) of Ireland, grant number: HRB MRCG 2008/1; website: http://www.hrb.ie/. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
JF  - PLoS One
VL  - 8
ER  -