eprintid: 1380126
rev_number: 46
eprint_status: archive
userid: 608
dir: disk0/01/38/01/26
datestamp: 2013-05-09 08:32:48
lastmod: 2019-10-19 08:37:00
status_changed: 2013-05-09 08:32:48
type: thesis
metadata_visibility: show
item_issues_count: 0
creators_name: Charbin, AA
title: The role of condensin in chromosome resolution
ispublished: unpub
divisions: A01
divisions: B02
divisions: C08
note: Copyright restricted material has been removed from the e-thesis
abstract: The condensin complex is a key determinant of mitotic chromosome architecture. In addition to its role in mitotic chromosome compaction, condensin is required for resolution of sister chromatid linkages during chromosome segregation in anaphase. How condensin resolves sister chromatids, and the nature of the chromosome bridges that are characteristic of cells harboring defective condensin, have remained topics of debate. Inactivation of topoisomerase II, the main enzyme that removes topological interlinks that persist between DNA replication products after their synthesis, leads to similar chromosome bridges. Here, we follow the catenation status of circular minichromosomes of three sizes during the S. cerevisiae cell cycle. Catenanes are produced in S-phase and, in part, they are readily resolved, aided by physical separation of sister chromatids during mitosis. Complete resolution, however, requires the condensin complex, a dependency that becomes more pronounced with increasing chromosome size. Condensin and topoisomerase II directly interact and, using purified proteins, we show that condensin stimulates DNA decatenation by topoisomerase II in vitro. Therefore, in parallel to promoting chromosome condensation, condensin facilitates topological resolution of sister chromatids to secure their successful segregation to daughter cells during cell division.
date: 2012-12-28
vfaculties: VFLS
oa_status: green
thesis_class: doctoral_open
language: eng
thesis_view: UCL_Thesis
dart: DART-Europe
primo: open
primo_central: open_green
verified: verified_manual
elements_source: Manually entered
elements_id: 837370
lyricists_name: Charbin, Adrian
lyricists_id: AACHA51
full_text_status: public
pagerange: ? - ?
pages: 152
institution: UCL (University College London)
department: Chromosome Segregation Laboratory
thesis_type: Doctoral
citation:        Charbin, AA;      (2012)    The role of condensin in chromosome resolution.                   Doctoral thesis , UCL (University College London).     Green open access   
 
document_url: https://discovery.ucl.ac.uk/id/eprint/1380126/4/Adrian_Charbin_2012_PhD_Thesis_No_3rd_Party_Images.pdf